Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida

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Kontturi , J , Osama , R , Deng , X , Bashandy , H , Albert , V A & Teeri , T H 2017 , ' Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida ' , Phytochemistry , vol. 134 , pp. 38-45 . https://doi.org/10.1016/j.phytochem.2016.11.002

Title: Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida
Author: Kontturi, Juha; Osama, Raisa; Deng, Xianbao; Bashandy, Hany; Albert, Victor A.; Teeri, Teemu H.
Contributor organization: University of Helsinki
Department of Agricultural Sciences
Teemu Teeri / Principal Investigator
Viikki Plant Science Centre (ViPS)
Asteraceae developmental biology and secondary metabolism
Plant Production Sciences
Date: 2017-02
Language: eng
Number of pages: 8
Belongs to series: Phytochemistry
ISSN: 0031-9422
DOI: https://doi.org/10.1016/j.phytochem.2016.11.002
URI: http://hdl.handle.net/10138/201880
Abstract: The chalcone synthase superfamily consists of type III polyketidesynthases (PKSs), enzymes responsible for producing plant secondary metabolites with various biological and pharmacological activities. Anther-specific chalcone synthase-like enzymes (ASCLs) represent an ancient group of type III PKSs involved in the biosynthesis of sporopollenin, the main component of the exine layer of moss spores and mature pollen grains of seed plants. In the latter, ASCL proteins are localized in the tapetal cells of the anther where they participate in sporopollenin biosynthesis and exine formation within the locule. It is thought that the enzymes responsible for sporopollenin biosynthesis are highly conserved, and thus far, each angiosperm species with a genome sequenced has possessed two ASCL genes, which in Arabidopsis thaliana are PKSA and PKSB. The Gerbera hybrida (gerbera) PKS protein family consists of three chalcone synthases (GCHS1, GCHS3 and GCHS4) and three 2-pyrone synthases (G2PS1, G2PS2 and G2PS3). In previous studies we have demonstrated the functions of chalcone synthases in flavonoid biosynthesis, and the involvement of 2-pyrone synthases in the biosynthesis of antimicrobial compounds found in gerbera. In this study we expanded the gerbera PKS-family by functionally characterizing two gerbera ASCL proteins. In vitro enzymatic studies using purified recombinant proteins showed that both GASCL1 and GASCL2 were able to use medium and long-chain acyl-CoA starters and perform two to three condensation reactions of malonyl-CoA to produce tri- and tetraketide 2-pyrones, usually referred to as alpha-pyrones in sporopollenin literature. Both GASCL1 and GASCL2 genes were expressed only floral organs, with most expression observed in anthers. In the anthers, transcripts of both genes showed strict tapetum-specific localization. (C) 2016 Elsevier Ltd. All rights reserved.
Subject: Gerbera hybrida
Asteraceae
Type III polyketide synthase
ASCL
Sporopollenin
III POLYKETIDE SYNTHASES
POLLEN EXINE DEVELOPMENT
SPOROPOLLENIN BIOSYNTHESIS
ARABIDOPSIS-THALIANA
ASTERACEAE INFLORESCENCE
ANTHOCYANIN BIOSYNTHESIS
GENES
DIVERSIFICATION
PATHWAY
PLANTS
1182 Biochemistry, cell and molecular biology
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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