Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography

Show full item record



Permalink

http://hdl.handle.net/10138/203728

Citation

Nieminen , H J , Ylitalo , T , Karhula , S , Suuronen , J -P , Kauppinen , S , Serimaa , R , Haeggstrom , E , Pritzker , K P H , Valkealahti , M , Lehenkari , P , Finnila , M & Saarakkala , S 2015 , ' Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography ' , Osteoarthritis and Cartilage , vol. 23 , no. 9 , pp. 1613-1621 . https://doi.org/10.1016/j.joca.2015.05.004

Title: Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography
Author: Nieminen, H. J.; Ylitalo, T.; Karhula, S.; Suuronen, J. -P.; Kauppinen, S.; Serimaa, R.; Haeggstrom, E.; Pritzker, K. P. H.; Valkealahti, M.; Lehenkari, P.; Finnila, M.; Saarakkala, S.
Contributor: University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
Date: 2015-09
Language: eng
Number of pages: 9
Belongs to series: Osteoarthritis and Cartilage
ISSN: 1063-4584
URI: http://hdl.handle.net/10138/203728
Abstract: Objective: Collagen distribution within articular cartilage (AC) is typically evaluated from histological sections, e.g., using collagen staining and light microscopy (LM). Unfortunately, all techniques based on histological sections are time-consuming, destructive, and without extraordinary effort, limited to two dimensions. This study investigates whether phosphotungstic acid (PTA) and phosphomolybdic acid (PMA), two collagen-specific markers and X-ray absorbers, could (1) produce contrast for AC X-ray imaging or (2) be used to detect collagen distribution within AC. Method: We labeled equine AC samples with PTA or PMA and imaged them with micro-computed tomography (micro-CT) at pre-defined time points 0, 18, 36, 54, 72, 90, 180, 270 h during staining. The micro-CT image intensity was compared with collagen distributions obtained with a reference technique, i.e., Fourier-transform infrared imaging (FTIRI). The labeling time and contrast agent producing highest association (Pearson correlation, BlandeAltman analysis) between FTIRI collagen distribution and micro-CT -determined PTA distribution was selected for human AC. Results: Both, PTA and PMA labeling permitted visualization of AC features using micro-CT in non-calcified cartilage. After labeling the samples for 36 h in PTA, the spatial distribution of X-ray attenuation correlated highly with the collagen distribution determined by FTIRI in both equine (mean +/- S.D. of the Pearson correlation coefficients, r = 0.96 +/- 0.03, n = 12) and human AC (r = 0.82 +/- 0.15, n = 4). Conclusions: PTA-induced X-ray attenuation is a potential marker for non-destructive detection of AC collagen distributions in 3D. This approach opens new possibilities in development of non-destructive 3D histopathological techniques for characterization of OA. (C) 2015 The Authors. Published by Elsevier Ltd and Osteoarthritis Research Society International.
Subject: Osteoarthritis
Imaging
Collagen
X-ray
Tomography
Articular cartilage
HUMAN KNEE
CHONDROCYTES
NANOPARTICLES
TISSUE
AGENT
DEATH
ACID
3126 Surgery, anesthesiology, intensive care, radiology
114 Physical sciences
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S1063458415011619_main.pdf 3.093Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record