Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography

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Nieminen , H J , Ylitalo , T , Karhula , S , Suuronen , J -P , Kauppinen , S , Serimaa , R , Haeggstrom , E , Pritzker , K P H , Valkealahti , M , Lehenkari , P , Finnila , M & Saarakkala , S 2015 , ' Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography ' , Osteoarthritis and Cartilage , vol. 23 , no. 9 , pp. 1613-1621 . https://doi.org/10.1016/j.joca.2015.05.004

Title: Determining collagen distribution in articular cartilage using contrast-enhanced micro-computed tomography
Author: Nieminen, H. J.; Ylitalo, T.; Karhula, S.; Suuronen, J. -P.; Kauppinen, S.; Serimaa, R.; Haeggstrom, E.; Pritzker, K. P. H.; Valkealahti, M.; Lehenkari, P.; Finnila, M.; Saarakkala, S.
Contributor organization: Department of Physics
Date: 2015-09
Language: eng
Number of pages: 9
Belongs to series: Osteoarthritis and Cartilage
ISSN: 1063-4584
DOI: https://doi.org/10.1016/j.joca.2015.05.004
URI: http://hdl.handle.net/10138/203728
Abstract: Objective: Collagen distribution within articular cartilage (AC) is typically evaluated from histological sections, e.g., using collagen staining and light microscopy (LM). Unfortunately, all techniques based on histological sections are time-consuming, destructive, and without extraordinary effort, limited to two dimensions. This study investigates whether phosphotungstic acid (PTA) and phosphomolybdic acid (PMA), two collagen-specific markers and X-ray absorbers, could (1) produce contrast for AC X-ray imaging or (2) be used to detect collagen distribution within AC. Method: We labeled equine AC samples with PTA or PMA and imaged them with micro-computed tomography (micro-CT) at pre-defined time points 0, 18, 36, 54, 72, 90, 180, 270 h during staining. The micro-CT image intensity was compared with collagen distributions obtained with a reference technique, i.e., Fourier-transform infrared imaging (FTIRI). The labeling time and contrast agent producing highest association (Pearson correlation, BlandeAltman analysis) between FTIRI collagen distribution and micro-CT -determined PTA distribution was selected for human AC. Results: Both, PTA and PMA labeling permitted visualization of AC features using micro-CT in non-calcified cartilage. After labeling the samples for 36 h in PTA, the spatial distribution of X-ray attenuation correlated highly with the collagen distribution determined by FTIRI in both equine (mean +/- S.D. of the Pearson correlation coefficients, r = 0.96 +/- 0.03, n = 12) and human AC (r = 0.82 +/- 0.15, n = 4). Conclusions: PTA-induced X-ray attenuation is a potential marker for non-destructive detection of AC collagen distributions in 3D. This approach opens new possibilities in development of non-destructive 3D histopathological techniques for characterization of OA. (C) 2015 The Authors. Published by Elsevier Ltd and Osteoarthritis Research Society International.
Subject: Osteoarthritis
Imaging
Collagen
X-ray
Tomography
Articular cartilage
HUMAN KNEE
CHONDROCYTES
NANOPARTICLES
TISSUE
AGENT
DEATH
ACID
3126 Surgery, anesthesiology, intensive care, radiology
114 Physical sciences
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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