A Strong Contractile Actin Fence and Large Adhesions Direct Human Pluripotent Colony Morphology and Adhesion

Show simple item record

dc.contributor.author Narva, Elisa
dc.contributor.author Stubb, Aki
dc.contributor.author Guzman, Camilo
dc.contributor.author Blomqvist, Matias
dc.contributor.author Balboa, Diego
dc.contributor.author Lerche, Martina
dc.contributor.author Saari, Markku
dc.contributor.author Otonkoski, Timo
dc.contributor.author Ivaska, Johanna
dc.date.accessioned 2017-07-28T11:33:01Z
dc.date.available 2017-07-28T11:33:01Z
dc.date.issued 2017-07-11
dc.identifier.citation Narva , E , Stubb , A , Guzman , C , Blomqvist , M , Balboa , D , Lerche , M , Saari , M , Otonkoski , T & Ivaska , J 2017 , ' A Strong Contractile Actin Fence and Large Adhesions Direct Human Pluripotent Colony Morphology and Adhesion ' , Stem cell reports , vol. 9 , no. 1 , pp. 67-76 . https://doi.org/10.1016/j.stemcr.2017.05.021
dc.identifier.other PURE: 87503416
dc.identifier.other PURE UUID: a7492822-18d3-43a1-9643-2a70da933a6f
dc.identifier.other WOS: 000405178400009
dc.identifier.other Scopus: 85020809055
dc.identifier.uri http://hdl.handle.net/10138/204689
dc.description.abstract Cell-type-specific functions and identity are tightly regulated by interactions between the cell cytoskeleton and the extracellular matrix (ECM). Human pluripotent stem cells (hPSCs) have ultimate differentiation capacity and exceptionally low-strength ECM contact, yet the organization and function of adhesion sites and associated actin cytoskeleton remain poorly defined. We imaged hPSCs at the cell-ECM interface with total internal reflection fluorescence microscopy and discovered that adhesions at the colony edge were exceptionally large and connected by thick ventral stress fibers. The actin fence encircling the colony was found to exert extensive Rho-ROCK-myosin-dependent mechanical stress to enforce colony morphology, compaction, and pluripotency and to define mitotic spindle orientation. Remarkably, differentiation altered adhesion organization and signaling characterized by a switch from ventral to dorsal stress fibers, reduced mechanical stress, and increased integrin activity and cell-ECM adhesion strength. Thus, pluripotency appears to be linked to unique colony organization and adhesion structure. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Stem cell reports
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject SELF-RENEWAL
dc.subject INTEGRIN
dc.subject SRC
dc.subject RHO
dc.subject CULTURE
dc.subject EXPANSION
dc.subject 3111 Biomedicine
dc.title A Strong Contractile Actin Fence and Large Adhesions Direct Human Pluripotent Colony Morphology and Adhesion en
dc.type Article
dc.contributor.organization University of Helsinki
dc.contributor.organization Research Programme for Molecular Neurology
dc.contributor.organization Research Programs Unit
dc.contributor.organization Timo Pyry Juhani Otonkoski / Principal Investigator
dc.contributor.organization Clinicum
dc.contributor.organization Children's Hospital
dc.contributor.organization HUS Children and Adolescents
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.stemcr.2017.05.021
dc.relation.issn 2213-6711
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S221367111730228X_main.pdf 4.839Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record