A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana

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Nowell , RW , Elsworth , B , Oostra , V , Zwaan , B J , Wheat , C W , Saastamoinen , M A K , Saccheri , I , Van't Hof , AE , Wasik , BR , Connahs , H , Kumar , S , Challis , RJ , Aslam , L , Monteiro , A , Brakefield , P M & Blaxter , M 2017 , ' A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana ' , GigaScience , vol. 6 , pp. 1-7 . https://doi.org/10.1093/gigascience/gix035

Titel: A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana
Författare: Nowell, RW; Elsworth, B; Oostra, Vicencio; Zwaan, Bas J.; Wheat, Christopher West; Saastamoinen, Marjo Anna Kaarina; Saccheri, Ilik; Van't Hof, AE; Wasik, BR; Connahs, H; Kumar, S; Challis, RJ; Aslam, L; Monteiro, Antonia; Brakefield, Paul M.; Blaxter, M
Upphovmannens organisation: Biosciences
Centre of Excellence in Metapopulation Research
Ecology and Evolutionary Biology
Life-history Evolution Research Group
Datum: 2017
Språk: eng
Sidantal: 7
Tillhör serie: GigaScience
ISSN: 2047-217X
DOI: https://doi.org/10.1093/gigascience/gix035
Permanenta länken (URI): http://hdl.handle.net/10138/205641
Abstrakt: The mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence for Bicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).
Subject: 1181 Ecology, evolutionary biology
Referentgranskad: Ja
Licens: cc_by
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion

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