Genomic RNA folding mediates assembly of human parechovirus

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Shakeel , S , Dykeman , E C , White , S J , Ora , A , Cockburn , J , Butcher , S J , Stockley , P G & Twarock , R 2017 , ' Genomic RNA folding mediates assembly of human parechovirus ' , Nature Communications , vol. 8 , no. 5 , 5 . https://doi.org/10.1038/s41467-016-0011-z

Title: Genomic RNA folding mediates assembly of human parechovirus
Author: Shakeel, Shabih; Dykeman, Eric C; White, Simon J; Ora, Ari; Cockburn, Joe; Butcher, Sarah Jane; Stockley, Peter G; Twarock, Reidun
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Biosciences
Date: 2017-02-23
Language: eng
Number of pages: 11
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/206651
Abstract: Assembly of the major viral pathogens of the Picornaviridae family is poorly understood. Human parechovirus 1 is an example of such viruses that contains 60 short regions of ordered RNA density making identical contacts with the protein shell. We show here via a combination of RNA-based systematic evolution of ligands by exponential enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to the coat proteins in a sequence-specific manner. Disruption of either the RNA coat protein recognition motif or its contact amino acid residues is deleterious for viral assembly. The data are consistent with RNA packaging signals playing essential roles in virion assembly. Their binding sites on the coat proteins are evolutionarily conserved across the Parechovirus genus, suggesting that they represent potential broad-spectrum anti-viral targets.
Subject: 1183 Plant biology, microbiology, virology
PARECHOVIRUS
VIRUS ASSEMBLY
SELEX
BIOINFORMATICS
STRUCTURAL BIOLOGY
PACKAGING SIGNALS
1182 Biochemistry, cell and molecular biology
Capsid assembly
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