Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9

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http://hdl.handle.net/10138/207005

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Balboa , D , Weltner , J , Novik , Y , Eurola , S , Wartiovaara , K & Otonkoski , T 2017 , ' Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9 ' , Stem Cell Research , vol. 22 , pp. 16-19 . https://doi.org/10.1016/j.scr.2017.05.005

Title: Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9
Author: Balboa, Diego; Weltner, Jere; Novik, Yuval; Eurola, Solja; Wartiovaara, Kirmo; Otonkoski, Timo
Contributor: University of Helsinki, Research Programme for Molecular Neurology
University of Helsinki, Research Programs Unit
University of Helsinki, Research Programs Unit
University of Helsinki, HUSLAB
University of Helsinki, University of Helsinki
Date: 2017-07
Language: eng
Number of pages: 4
Belongs to series: Stem Cell Research
ISSN: 1873-5061
URI: http://hdl.handle.net/10138/207005
Abstract: SOX2 is an important transcription factor involved in pluripotency maintenance, pluripotent reprogramming and differentiation towards neural lineages. Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette before the STOP codon of the SOX2 gene coding sequence. CRISPR/SaCas9-mediated stimulation of homologous recombination was utilized to facilitate faithful targeted insertion. This line accurately reports the expression of endogenous SOX2 and therefore constitutes a useful tool to study the SOX2 expression dynamics upon hiPSC culture, differentiation and somatic cell reprogramming. (C) 2017 The Authors. Published by Elsevier B.V.
Subject: 1182 Biochemistry, cell and molecular biology
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