Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential

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Kyttala , A , Moraghebi , R , Valensisi , C , Kettunen , J , Andrus , C , Pasumarthy , K K , Nakanishi , M , Nishimura , K , Ohtaka , M , Weltner , J , Van Handel , B , Parkkonen , O , Sinisalo , J , Jalanko , A , Hawkins , R D , Woods , N-B , Otonkoski , T & Trokovic , R 2016 , ' Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential ' , Stem cell reports , vol. 6 , no. 2 , pp. 200-212 . https://doi.org/10.1016/j.stemcr.2015.12.009

Title: Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential
Author: Kyttala, Aija; Moraghebi, Roksana; Valensisi, Cristina; Kettunen, Johannes; Andrus, Colin; Pasumarthy, Kalyan Kumar; Nakanishi, Mahito; Nishimura, Ken; Ohtaka, Manami; Weltner, Jere; Van Handel, Ben; Parkkonen, Olavi; Sinisalo, Juha; Jalanko, Anu; Hawkins, R. David; Woods, Niels-Bjarne; Otonkoski, Timo; Trokovic, Ras
Contributor organization: Research Programs Unit
Timo Pyry Juhani Otonkoski / Principal Investigator
Research Programme for Molecular Neurology
Clinicum
Department of Medicine
Kardiologian yksikkö
Children's Hospital
HUS Children and Adolescents
Date: 2016-02-09
Language: eng
Number of pages: 13
Belongs to series: Stem cell reports
ISSN: 2213-6711
DOI: https://doi.org/10.1016/j.stemcr.2015.12.009
URI: http://hdl.handle.net/10138/208095
Abstract: Reports on the retention of somatic cell memory in induced pluripotent stem cells (iPSCs) have complicated the selection of the optimal cell type for the generation of iPSC biobanks. To address this issue we compared transcriptomic, epigenetic, and differentiation propensities of genetically matched human iPSCs derived from fibroblasts and blood, two tissues of the most practical relevance for biobanking. Our results show that iPSC lines derived from the same donor are highly similar to each other. However, genetic variation imparts a donor-specific expression and methylation profile in reprogrammed cells that leads to variable functional capacities of iPSC lines. Our results suggest that integration-free, bona fide iPSC lines from fibroblasts and blood can be combined in repositories to form biobanks. Due to the impact of genetic variation on iPSC differentiation, biobanks should contain cells from large numbers of donors.
Subject: PLURIPOTENT STEM-CELLS
HIGH-THROUGHPUT DATA
EPIGENETIC MEMORY
EFFICIENT GENERATION
LINES
REPRESENTATION
HETEROGENEITY
TRANSCRIPTION
MICROARRAYS
WIDESPREAD
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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