Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells

Show full item record



Permalink

http://hdl.handle.net/10138/208100

Citation

Salmiheimo , A N E , Mustonen , H K , Vainionpaa , S A A , Shen , Z , Kemppainen , E A J , Seppanen , H E & Puolakkainen , P A 2016 , ' Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells ' Journal of Cancer , vol. 7 , no. 1 , pp. 42-49 . DOI: 10.7150/jca.12923

Title: Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells
Author: Salmiheimo, Aino N. E.; Mustonen, Harri K.; Vainionpaa, Sanna A. A.; Shen, Zhanlong; Kemppainen, Esko A. J.; Seppanen, Hanna E.; Puolakkainen, Pauli A.
Contributor: University of Helsinki, Department of Surgery
University of Helsinki, II kirurgian klinikka
University of Helsinki, Department of Surgery
University of Helsinki, Department of Surgery
Date: 2016
Language: eng
Number of pages: 8
Belongs to series: Journal of Cancer
ISSN: 1837-9664
URI: http://hdl.handle.net/10138/208100
Abstract: Recent studies suggest that pro-inflammatory type M1 macrophages inhibit tumor progression and that anti-inflammatory M2 macrophages enhance it. The aim of this study was to examine the interaction of type M1 and M2 macrophages with pancreatic cancer cells. We studied the migration rate of fluorescein stained pancreatic cancer cells on Matrigel cultured alone or with Granulocyte- Macrophage Colony Stimulating Factor (GM-CSF) differentiated macrophages or with Macrophage Colony Stimulating Factor (M-CSF) differentiated macrophages, skewing the phenotype towards pro- and anti-inflammatory direction, respectively. Macrophage differentiation was assessed with flow cytometry and the cytokine secretion in cell cultures with cytokine array. Both GM-CSF and M-CSF differentiated macrophages increased the migration rate of primary pancreatic adenocarcinoma cell line (MiaPaCa-2) and metastatic cell line (HPAF-II). Stimulation with IL6 or IL4+ LPS reversed the macrophages' increasing effect on the migration rate of Mi-aPaCa-2 completely and partly of HPAF-II. Co-culture with MiaPaCa-2 reduced the inflammatory cytokine secretion of GM-CSF differentiated macrophages. Co-culture of macrophages with pancreatic cancer cells seem to change the inflammatory cytokine profile of GM-CSF differentiated macrophages and this might explain why also GM-CSF differentiated macrophages promoted the invasion. Adding IL6 or IL4+ LPS to the cell culture with MiaPaCa-2 and GM-CSF or M-CSF differentiated macrophages increased the secretion of inflammatory cytokines and this could contribute to the reversion of the macrophage induced increase of cancer cell migration rate.
Subject: pancreatic adenocarcinoma
M1
M2
macrophages
invasion
TUMOR-ASSOCIATED MACROPHAGES
ACTIVATED MACROPHAGES
ROLES
GROWTH
MICROENVIRONMENT
POLARIZATION
PROGRESSION
METASTASIS
PROFILES
PROMOTE
3122 Cancers
3126 Surgery, anesthesiology, intensive care, radiology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
v07p0042.pdf 646.2Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record