Innovative molecular diagnosis of Trichinella species based on β-carbonic anhydrase genomic sequence

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http://hdl.handle.net/10138/208102

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Emameh , R Z , Kuuslahti , M , Näreaho , A , Sukura , A & Parkkila , S 2016 , ' Innovative molecular diagnosis of Trichinella species based on β-carbonic anhydrase genomic sequence ' , Microbial Biotechnology , vol. 9 , no. 2 , pp. 172-179 . https://doi.org/10.1111/1751-7915.12327

Title: Innovative molecular diagnosis of Trichinella species based on β-carbonic anhydrase genomic sequence
Author: Emameh, Reza Zolfaghari; Kuuslahti, Marianne; Näreaho, Anu; Sukura, Antti; Parkkila, Seppo
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
Date: 2016-03
Language: eng
Number of pages: 8
Belongs to series: Microbial Biotechnology
ISSN: 1751-7907
URI: http://hdl.handle.net/10138/208102
Abstract: Trichinellosis is a helminthic infection where different species of Trichinella nematodes are the causative agents. Several molecular assays have been designed to aid diagnostics of trichinellosis. These assays are mostly complex and expensive. The genomes of Trichinella species contain certain parasite-specific genes, which can be detected by polymerase chain reaction (PCR) methods. We selected -carbonic anhydrase (-CA) gene as a target, because it is present in many parasites genomes but absent in vertebrates. We developed a novel -CA gene-based method for detection of Trichinella larvae in biological samples. We first identified a -CA protein sequence from Trichinella spiralis by bioinformatic tools using -CAs from Caenorhabditis elegans and Drosophila melanogaster. Thereafter, 16 sets of designed primers were tested to detect -CA genomic sequences from three species of Trichinella, including T.spiralis, Trichinellapseudospiralis and Trichinellanativa. Among all 16 sets of designed primers, the primer set No. 2 efficiently amplified -CA genomic sequences from T.spiralis, T.pseudospiralis and T.nativa without any false-positive amplicons from other parasite samples including Toxoplasma gondii, Toxocara cati and Parascaris equorum. This robust and straightforward method could be useful for meat inspection in slaughterhouses, quality control by food authorities and medical laboratories.
Subject: POLYMERASE-CHAIN-REACTION
MULTIPLEX PCR
TRICHINOSIS
INHIBITORS
SPIRALIS
IDENTIFICATION
INFECTION
ANIMALS
TARGET
ENZYME
413 Veterinary science
1183 Plant biology, microbiology, virology
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