Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure

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http://hdl.handle.net/10138/209596

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Deger , A G , Scherzer , S , Nuhkat , M , Kedzierska , J , Kollist , H , Brosche , M , Unyayar , S , Boudsocq , M , Hedrich , R & Roelfsema , M R G 2015 , ' Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure ' , New Phytologist , vol. 208 , no. 1 , pp. 162-173 . https://doi.org/10.1111/nph.13435

Title: Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure
Author: Deger, Aysin Guzel; Scherzer, Sönke; Nuhkat, Maris; Kedzierska, Justyna; Kollist, Hannes; Brosche, Mikael; Unyayar, Serpil; Boudsocq, Marie; Hedrich, Rainer; Roelfsema, M. Rob G.
Other contributor: University of Helsinki, University of Tartu
University of Helsinki, Biosciences



Date: 2015-10
Language: eng
Number of pages: 12
Belongs to series: New Phytologist
ISSN: 0028-646X
DOI: https://doi.org/10.1111/nph.13435
URI: http://hdl.handle.net/10138/209596
Abstract: During infection plants recognize microbe-associated molecular patterns (MAMPs), and this leads to stomatal closure. This study analyzes the molecular mechanisms underlying this MAMP response and its interrelation with ABA signaling. Stomata in intact Arabidopsis thaliana plants were stimulated with the bacterial MAMP flg22, or the stress hormone ABA, by using the noninvasive nanoinfusion technique. Intracellular double-barreled microelectrodes were applied to measure the activity of plasma membrane ion channels. Flg22 induced rapid stomatal closure and stimulated the SLAC1 and SLAH3 anion channels in guard cells. Loss of both channels resulted in cells that lacked flg22-induced anion channel activity and stomata that did not close in response to flg22 or ABA. Rapid flg22-dependent stomatal closure was impaired in plants that were flagellin receptor (FLS2)-deficient, as well as in the ost1-2 (Open Stomata 1) mutant, which lacks a key ABA-signaling protein kinase. By contrast, stomata of the ABA protein phosphatase mutant abi1-1 (ABscisic acid Insensitive 1) remained flg22-responsive. These data suggest that the initial steps in flg22 and ABA signaling are different, but that the pathways merge at the level of OST1 and lead to activation of SLAC1 and SLAH3 anion channels.
Subject: ABA
Arabidopsis thaliana
flg22
guard cells
innate immunity
microbe-associated molecular pattern (MAMP)
S-type anion channel
stomata
PLANT INNATE IMMUNITY
PATTERN-RECOGNITION RECEPTORS
BACTERIAL ELICITOR FLAGELLIN
DEPENDENT PROTEIN-KINASES
ABSCISIC-ACID SIGNAL
NADPH OXIDASE RBOHD
ARABIDOPSIS-THALIANA
REACTIVE OXYGEN
MOLECULAR-PATTERNS
DEFENSE RESPONSES
1183 Plant biology, microbiology, virology
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