Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure

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dc.contributor.author Deger, Aysin Guzel
dc.contributor.author Scherzer, Sönke
dc.contributor.author Nuhkat, Maris
dc.contributor.author Kedzierska, Justyna
dc.contributor.author Kollist, Hannes
dc.contributor.author Brosche, Mikael
dc.contributor.author Unyayar, Serpil
dc.contributor.author Boudsocq, Marie
dc.contributor.author Hedrich, Rainer
dc.contributor.author Roelfsema, M. Rob G.
dc.date.accessioned 2017-08-10T12:23:00Z
dc.date.available 2017-08-10T12:23:00Z
dc.date.issued 2015-10
dc.identifier.citation Deger , A G , Scherzer , S , Nuhkat , M , Kedzierska , J , Kollist , H , Brosche , M , Unyayar , S , Boudsocq , M , Hedrich , R & Roelfsema , M R G 2015 , ' Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure ' , New Phytologist , vol. 208 , no. 1 , pp. 162-173 . https://doi.org/10.1111/nph.13435
dc.identifier.other PURE: 54132755
dc.identifier.other PURE UUID: a132656c-309e-4594-8ce4-2097a6fbc174
dc.identifier.other WOS: 000360376400021
dc.identifier.other Scopus: 84940467380
dc.identifier.other ORCID: /0000-0002-1135-2496/work/28761842
dc.identifier.uri http://hdl.handle.net/10138/209596
dc.description.abstract During infection plants recognize microbe-associated molecular patterns (MAMPs), and this leads to stomatal closure. This study analyzes the molecular mechanisms underlying this MAMP response and its interrelation with ABA signaling. Stomata in intact Arabidopsis thaliana plants were stimulated with the bacterial MAMP flg22, or the stress hormone ABA, by using the noninvasive nanoinfusion technique. Intracellular double-barreled microelectrodes were applied to measure the activity of plasma membrane ion channels. Flg22 induced rapid stomatal closure and stimulated the SLAC1 and SLAH3 anion channels in guard cells. Loss of both channels resulted in cells that lacked flg22-induced anion channel activity and stomata that did not close in response to flg22 or ABA. Rapid flg22-dependent stomatal closure was impaired in plants that were flagellin receptor (FLS2)-deficient, as well as in the ost1-2 (Open Stomata 1) mutant, which lacks a key ABA-signaling protein kinase. By contrast, stomata of the ABA protein phosphatase mutant abi1-1 (ABscisic acid Insensitive 1) remained flg22-responsive. These data suggest that the initial steps in flg22 and ABA signaling are different, but that the pathways merge at the level of OST1 and lead to activation of SLAC1 and SLAH3 anion channels. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof New Phytologist
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject ABA
dc.subject Arabidopsis thaliana
dc.subject flg22
dc.subject guard cells
dc.subject innate immunity
dc.subject microbe-associated molecular pattern (MAMP)
dc.subject S-type anion channel
dc.subject stomata
dc.subject PLANT INNATE IMMUNITY
dc.subject PATTERN-RECOGNITION RECEPTORS
dc.subject BACTERIAL ELICITOR FLAGELLIN
dc.subject DEPENDENT PROTEIN-KINASES
dc.subject ABSCISIC-ACID SIGNAL
dc.subject NADPH OXIDASE RBOHD
dc.subject ARABIDOPSIS-THALIANA
dc.subject REACTIVE OXYGEN
dc.subject MOLECULAR-PATTERNS
dc.subject DEFENSE RESPONSES
dc.subject 1183 Plant biology, microbiology, virology
dc.title Guard cell SLAC1-type anion channels mediate flagellin-induced stomatal closure en
dc.type Article
dc.contributor.organization Biosciences
dc.contributor.organization Plant stress and natural variation
dc.contributor.organization Plant Biology
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/nph.13435
dc.relation.issn 0028-646X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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