Human corneal cell culture models for drug toxicity studies

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http://hdl.handle.net/10138/212982

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Rönkkö , S , Vellonen , K-S , Järvinen , K , Toropainen , E & Urtti , A 2016 , ' Human corneal cell culture models for drug toxicity studies ' , Drug Delivery and Translational Research , vol. 6 , no. 6 , pp. 660-675 . https://doi.org/10.1007/s13346-016-0330-y

Title: Human corneal cell culture models for drug toxicity studies
Author: Rönkkö, Seppo; Vellonen, Kati-Sisko; Järvinen, Kristiina; Toropainen, Elisa; Urtti, Arto
Contributor: University of Helsinki, University of Eastern Finland (UEF)
University of Helsinki, Faculty of Pharmacy
Date: 2016-12
Language: eng
Number of pages: 16
Belongs to series: Drug Delivery and Translational Research
ISSN: 2190-393X
URI: http://hdl.handle.net/10138/212982
Abstract: In vivo toxicity and absorption studies of topical ocular drugs are problematic, because these studies involve invasive tissue sampling and toxic effects in animal models. Therefore, different human corneal models ranging from simple monolayer cultures to three-dimensional models have been developed for toxicological prediction with in vitro models. Each system has its own set of advantages and disadvantages. Use of non-corneal cells, inadequate characterization of gene-expression profiles, and accumulation of genomic aberrations in human corneal models are typical drawbacks that decrease their reliability and predictive power. In the future, further improvements are needed for verifying comparable expression profiles and cellular properties of human corneal models with their in vivo counterparts. A rapidly expanding stem cell technology combined with tissue engineering may give future opportunities to develop new tools in drug toxicity studies. One approach may be the production of artificial miniature corneas. In addition, there is also a need to use large-scale profiling approaches such as genomics, transcriptomics, proteomics, and metabolomics for understanding of the ocular toxicity.
Subject: Ocular toxicity
Corneal cell culture
ADME prediction
In vitro model
Ocular bioavailability
EPITHELIAL STEM-CELLS
VITRO TISSUE MODELS
EYE IRRITATION TEST
HCE TEST METHOD
IN-VITRO
ENDOTHELIAL-CELLS
AMNIOTIC MEMBRANE
PROTEIN EXPRESSION
OCULAR IRRITATION
ABC TRANSPORTERS
317 Pharmacy
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