A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair

Show full item record



Permalink

http://hdl.handle.net/10138/223860

Citation

Ainola , M , Tomaszewski , W , Ostrowska , B , Wesolowska , E , Wagner , H D , Swieszkowski , W , Sillat , T , Peltola , E & Konttinen , Y T 2016 , ' A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair ' , Journal of Biomaterials Applications , vol. 30 , no. 6 , pp. 873-885 . https://doi.org/10.1177/0885328215604069

Title: A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair
Author: Ainola, Mari; Tomaszewski, Waclaw; Ostrowska, Barbara; Wesolowska, Ewa; Wagner, H. Daniel; Swieszkowski, Wojciech; Sillat, Tarvo; Peltola, Emilia; Konttinen, Yrjo T.
Contributor: University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2016-01
Language: eng
Number of pages: 13
Belongs to series: Journal of Biomaterials Applications
ISSN: 0885-3282
URI: http://hdl.handle.net/10138/223860
Abstract: The aim was to develop a hybrid three-dimensional-tissue engineering construct for chondrogenesis. The hypothesis was that they support chondrogenesis. A biodegradable, highly porous polycaprolactone-grate was produced by solid freeform fabrication. The polycaprolactone support was coated with a chitosan/polyethylene oxide nanofibre sheet produced by electrospinning. Transforming growth factor-3-induced chondrogenesis was followed using the following markers: sex determining region Y/-box 9, runt-related transcription factor 2 and collagen II and X in quantitative real-time polymerase chain reaction, histology and immunostaining. A polycaprolactone-grate and an optimized chitosan/polyethylene oxide nanofibre sheet supported cellular aggregation, chondrogenesis and matrix formation. In tissue engineering constructs, the sheets were seeded first with mesenchymal stem cells and then piled up according to the lasagne principle. The advantages of such a construct are (1) the cells do not need to migrate to the tissue engineering construct and therefore pore size and interconnectivity problems are omitted and (2) the cell-tight nanofibre sheet and collagen-fibre network mimic a cell culture platform for mesenchymal stem cells/chondrocytes (preventing escape) and hinders in-growth of fibroblasts and fibrous scarring (preventing capture). This allows time for the slowly progressing, multiphase true cartilage regeneration.
Subject: Scaffold
cartilage
chondrogenesis
rapid prototyping
nanofibres
chitosan
AUTOLOGOUS CHONDROCYTE TRANSPLANTATION
MESENCHYMAL STEM-CELLS
NANOFIBERS
DEFECTS
CHONDROGENESIS
CHITOSAN
KNEE
REGENERATION
SCAFFOLDS
BONE
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
A_bioactive_hybrid.pdf 1.353Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record