Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix

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Kanninen , L K , Porola , P , Niklander , J , Malinen , M M , Corlu , A , Guguen-Guillouzo , C , Urtti , A , Yliperttula , M L & Lou , Y-R 2016 , ' Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix ' , Experimental Cell Research , vol. 341 , no. 2 , pp. 207-217 . https://doi.org/10.1016/j.yexcr.2016.02.006

Title: Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix
Author: Kanninen, Liisa K.; Porola, Pauliina; Niklander, Johanna; Malinen, Melina M.; Corlu, Anne; Guguen-Guillouzo, Christiane; Urtti, Arto; Yliperttula, Marjo L.; Lou, Yan-Ru
Other contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy







Date: 2016-02-15
Language: eng
Number of pages: 11
Belongs to series: Experimental Cell Research
ISSN: 0014-4827
DOI: https://doi.org/10.1016/j.yexcr.2016.02.006
URI: http://hdl.handle.net/10138/223899
Abstract: Human hepatocytes are extensively needed in drug discovery and development. Stem cell-derived hepatocytes are expected to be an improved and continuous model of human liver to study drug candidates. Generation of endoderm-derived hepatocytes from human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, is a complex, challenging process requiring specific signals from soluble factors and insoluble matrices at each developmental stage. In this study, we used human liver progenitor HepaRG-derived acellular matrix (ACM) as a hepatic progenitor-specific matrix to induce hepatic commitment of hPSC-derived definitive endoderm (DE) cells. The DE cells showed much better attachment to the HepaRG ACM than other matrices tested and then differentiated towards hepatic cells, which expressed hepatocyte-specific makers. We demonstrate that Matrigel overlay induced hepatocyte phenotype and inhibited biliary epithelial differentiation in two hPSC lines studied. In conclusion, our study demonstrates that the HepaRG ACM, a hepatic progenitor-specific matrix, plays an important role in the hepatic differentiation of hPSCs. (C) 2016 Elsevier Inc. All rights reserved.
Subject: Extracellular matrix
Acellular matrix
Human embryonic stem cell
Human induced pluripotent stem cell
Hepatic differentiation
HepaRG
HEPATOCYTE-LIKE CELLS
DEFINITIVE ENDODERM CELLS
EXTRACELLULAR-MATRIX
EFFICIENT DIFFERENTIATION
3-DIMENSIONAL CULTURE
TRANSCRIPTION FACTORS
SODIUM-BUTYRATE
EXPRESSION
GROWTH
FETAL
317 Pharmacy
1182 Biochemistry, cell and molecular biology
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