Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix

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dc.contributor.author Kanninen, Liisa K.
dc.contributor.author Porola, Pauliina
dc.contributor.author Niklander, Johanna
dc.contributor.author Malinen, Melina M.
dc.contributor.author Corlu, Anne
dc.contributor.author Guguen-Guillouzo, Christiane
dc.contributor.author Urtti, Arto
dc.contributor.author Yliperttula, Marjo L.
dc.contributor.author Lou, Yan-Ru
dc.date.accessioned 2017-09-14T09:51:50Z
dc.date.available 2021-12-17T18:49:21Z
dc.date.issued 2016-02-15
dc.identifier.citation Kanninen , L K , Porola , P , Niklander , J , Malinen , M M , Corlu , A , Guguen-Guillouzo , C , Urtti , A , Yliperttula , M L & Lou , Y-R 2016 , ' Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix ' , Experimental Cell Research , vol. 341 , no. 2 , pp. 207-217 . https://doi.org/10.1016/j.yexcr.2016.02.006
dc.identifier.other PURE: 61597941
dc.identifier.other PURE UUID: 478e7e89-5023-41ef-b436-4820672be376
dc.identifier.other WOS: 000371446700011
dc.identifier.other Scopus: 84958911039
dc.identifier.other ORCID: /0000-0001-7717-6010/work/29111201
dc.identifier.uri http://hdl.handle.net/10138/223899
dc.description.abstract Human hepatocytes are extensively needed in drug discovery and development. Stem cell-derived hepatocytes are expected to be an improved and continuous model of human liver to study drug candidates. Generation of endoderm-derived hepatocytes from human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, is a complex, challenging process requiring specific signals from soluble factors and insoluble matrices at each developmental stage. In this study, we used human liver progenitor HepaRG-derived acellular matrix (ACM) as a hepatic progenitor-specific matrix to induce hepatic commitment of hPSC-derived definitive endoderm (DE) cells. The DE cells showed much better attachment to the HepaRG ACM than other matrices tested and then differentiated towards hepatic cells, which expressed hepatocyte-specific makers. We demonstrate that Matrigel overlay induced hepatocyte phenotype and inhibited biliary epithelial differentiation in two hPSC lines studied. In conclusion, our study demonstrates that the HepaRG ACM, a hepatic progenitor-specific matrix, plays an important role in the hepatic differentiation of hPSCs. (C) 2016 Elsevier Inc. All rights reserved. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Experimental Cell Research
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Extracellular matrix
dc.subject Acellular matrix
dc.subject Human embryonic stem cell
dc.subject Human induced pluripotent stem cell
dc.subject Hepatic differentiation
dc.subject HepaRG
dc.subject EXPRESSION
dc.subject GROWTH
dc.subject FETAL
dc.subject 317 Pharmacy
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix en
dc.type Article
dc.contributor.organization Faculty of Pharmacy
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.contributor.organization Nanobio Pharmaceutics
dc.contributor.organization Drug Delivery
dc.contributor.organization Drug Research Program
dc.contributor.organization Tissue engineering for drug research
dc.contributor.organization Drug Delivery Unit
dc.contributor.organization Biopharmaceutics Group
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.yexcr.2016.02.006
dc.relation.issn 0014-4827
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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