MicroRNA-192*impairs adipocyte triglyceride storage

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Pysyväisosoite

http://hdl.handle.net/10138/223915

Lähdeviite

Mysore , R , Zhou , Y , Sädevirta , S , Savolainen-Peltonen , H , Haridas , P A N , Soronen , J , Leivonen , M , Sarin , A-P , Fischer-Posovszky , P , Wabitsch , M , Yki-Jarvinen , H & Olkkonen , V M 2016 , ' MicroRNA-192*impairs adipocyte triglyceride storage ' , Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids , vol. 1861 , no. 4 , pp. 342-351 . https://doi.org/10.1016/j.bbalip.2015.12.019

Julkaisun nimi: MicroRNA-192*impairs adipocyte triglyceride storage
Tekijä: Mysore, Raghavendra; Zhou, You; Sädevirta, Sanja; Savolainen-Peltonen, Hanna; Haridas, P. A. Nidhina; Soronen, Jarkko; Leivonen , Marja; Sarin, Antti-Pekka; Fischer-Posovszky, Pamela; Wabitsch, Martin; Yki-Jarvinen, Hannele; Olkkonen, Vesa M.
Tekijän organisaatio: Department of Medicine
Clinicum
HUS Gynecology and Obstetrics
II kirurgian klinikka
Institute for Molecular Medicine Finland
Hannele Yki-Järvinen Research Group
Medicum
Department of Anatomy
Päiväys: 2016-04
Kieli: eng
Sivumäärä: 10
Kuuluu julkaisusarjaan: Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids
ISSN: 1388-1981
DOI-tunniste: https://doi.org/10.1016/j.bbalip.2015.12.019
URI: http://hdl.handle.net/10138/223915
Tiivistelmä: We investigated the expression of miR-192* (miR-192-3p) in the visceral adipose tissue (VAT) of obese subjects and its function in cultured human adipocytes. This miRNA is a 3' arm derived from the same pre-miRNA as miR-192 (miR-192-5p) implicated in type 2 diabetes, liver disease and cancers, and is predicted to target key genes in lipid metabolism. In morbidly obese subjects undergoing bariatric surgery preceded by a very low calorie diet, miR-192* in VAT correlated negatively (r = -0.387; p = 0.046) with serum triglyceride (TG) and positively with high-density lipoprotein (HDL) concentration (r = 0.396; p = 0.041). In a less obese patient cohort, the miRNA correlated negatively with the body mass index (r = -0.537; p = 0.026). To characterize the function of miR-192*, we overexpressed it in cultured adipocytes and analyzed the expression of adipogenic differentiation markers as well as cellular TG content. Reduced TG and expression of the adipocyte marker proteins aP2 (adipocyte protein 2) and perilipin 1 were observed. The function of miR-192* was further investigated by transcriptomic profiling of adipocytes expressing this miRNA, revealing impacts on key lipogenic genes. A number of the mRNA alterations were validated by qPCR. Western analysis confirmed a marked reduction of the lipogenic enzyme SCD (stearoyl coenzyme A desaturase-1), the fatty aldehyde dehydrogenase ALDH3A2 (aldehyde dehydrogenase 3 family member A2) and the high-density lipoprotein receptor SCARB1 (scavenger receptor B, type I). SCD and ALDH3A2 were demonstrated to be direct targets of miR-192*. To conclude, the present data identify miR-192* as a novel controller of adipocyte differentiation and lipid homeostasis. (C) 2016 Elsevier B.V. All rights reserved.
Avainsanat: Adipogenesis
miR-192-3p
Obesity
SGBS
Transcriptome
STEAROYL-COA DESATURASE-1
CARDIOVASCULAR-DISEASE
ADIPOSE-TISSUE
GENE-EXPRESSION
PROTECTS MICE
LIVER-DISEASE
DIFFERENTIATION
MICRORNAS
OBESITY
RISK
3111 Biomedicine
Vertaisarvioitu: Kyllä
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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