MicroRNA-192*impairs adipocyte triglyceride storage

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dc.contributor.author Mysore, Raghavendra
dc.contributor.author Zhou, You
dc.contributor.author Sädevirta, Sanja
dc.contributor.author Savolainen-Peltonen, Hanna
dc.contributor.author Haridas, P. A. Nidhina
dc.contributor.author Soronen, Jarkko
dc.contributor.author Leivonen , Marja
dc.contributor.author Sarin, Antti-Pekka
dc.contributor.author Fischer-Posovszky, Pamela
dc.contributor.author Wabitsch, Martin
dc.contributor.author Yki-Jarvinen, Hannele
dc.contributor.author Olkkonen, Vesa M.
dc.date.accessioned 2017-09-14T09:52:07Z
dc.date.available 2021-12-17T18:48:48Z
dc.date.issued 2016-04
dc.identifier.citation Mysore , R , Zhou , Y , Sädevirta , S , Savolainen-Peltonen , H , Haridas , P A N , Soronen , J , Leivonen , M , Sarin , A-P , Fischer-Posovszky , P , Wabitsch , M , Yki-Jarvinen , H & Olkkonen , V M 2016 , ' MicroRNA-192*impairs adipocyte triglyceride storage ' , Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids , vol. 1861 , no. 4 , pp. 342-351 . https://doi.org/10.1016/j.bbalip.2015.12.019
dc.identifier.other PURE: 61947907
dc.identifier.other PURE UUID: 9495bf96-c618-4776-8dd7-1daef97027e7
dc.identifier.other WOS: 000371940300008
dc.identifier.other Scopus: 84957606177
dc.identifier.other ORCID: /0000-0003-4139-669X/work/43908102
dc.identifier.uri http://hdl.handle.net/10138/223915
dc.description.abstract We investigated the expression of miR-192* (miR-192-3p) in the visceral adipose tissue (VAT) of obese subjects and its function in cultured human adipocytes. This miRNA is a 3' arm derived from the same pre-miRNA as miR-192 (miR-192-5p) implicated in type 2 diabetes, liver disease and cancers, and is predicted to target key genes in lipid metabolism. In morbidly obese subjects undergoing bariatric surgery preceded by a very low calorie diet, miR-192* in VAT correlated negatively (r = -0.387; p = 0.046) with serum triglyceride (TG) and positively with high-density lipoprotein (HDL) concentration (r = 0.396; p = 0.041). In a less obese patient cohort, the miRNA correlated negatively with the body mass index (r = -0.537; p = 0.026). To characterize the function of miR-192*, we overexpressed it in cultured adipocytes and analyzed the expression of adipogenic differentiation markers as well as cellular TG content. Reduced TG and expression of the adipocyte marker proteins aP2 (adipocyte protein 2) and perilipin 1 were observed. The function of miR-192* was further investigated by transcriptomic profiling of adipocytes expressing this miRNA, revealing impacts on key lipogenic genes. A number of the mRNA alterations were validated by qPCR. Western analysis confirmed a marked reduction of the lipogenic enzyme SCD (stearoyl coenzyme A desaturase-1), the fatty aldehyde dehydrogenase ALDH3A2 (aldehyde dehydrogenase 3 family member A2) and the high-density lipoprotein receptor SCARB1 (scavenger receptor B, type I). SCD and ALDH3A2 were demonstrated to be direct targets of miR-192*. To conclude, the present data identify miR-192* as a novel controller of adipocyte differentiation and lipid homeostasis. (C) 2016 Elsevier B.V. All rights reserved. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Adipogenesis
dc.subject miR-192-3p
dc.subject Obesity
dc.subject SGBS
dc.subject Transcriptome
dc.subject STEAROYL-COA DESATURASE-1
dc.subject CARDIOVASCULAR-DISEASE
dc.subject ADIPOSE-TISSUE
dc.subject GENE-EXPRESSION
dc.subject PROTECTS MICE
dc.subject LIVER-DISEASE
dc.subject DIFFERENTIATION
dc.subject MICRORNAS
dc.subject OBESITY
dc.subject RISK
dc.subject 3111 Biomedicine
dc.title MicroRNA-192*impairs adipocyte triglyceride storage en
dc.type Article
dc.contributor.organization Department of Medicine
dc.contributor.organization Clinicum
dc.contributor.organization HUS Gynecology and Obstetrics
dc.contributor.organization II kirurgian klinikka
dc.contributor.organization Institute for Molecular Medicine Finland
dc.contributor.organization Hannele Yki-Järvinen Research Group
dc.contributor.organization Medicum
dc.contributor.organization Department of Anatomy
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.bbalip.2015.12.019
dc.relation.issn 1388-1981
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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