Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding

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Bjorkgren , I , Alvarez , L , Blank , N , Balbach , M , Turunen , H , Laajala , T D , Toivanen , J , Krutskikh , A , Wahlberg , N , Huhtaniemi , I , Poutanen , M , Wachten , D & Sipila , P 2016 , ' Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding ' , Molecular and Cellular Endocrinology , vol. 427 , no. C , pp. 143-154 . https://doi.org/10.1016/j.mce.2016.03.013

Title: Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding
Author: Bjorkgren, Ida; Alvarez, Luis; Blank, Nelli; Balbach, Melanie; Turunen, Heikki; Laajala, Teemu Daniel; Toivanen, Jussi; Krutskikh, Anton; Wahlberg, Niklas; Huhtaniemi, Ilpo; Poutanen, Matti; Wachten, Dagmar; Sipila, Petra
Other contributor: University of Helsinki, Institute for Molecular Medicine Finland
Date: 2016-05-15
Language: eng
Number of pages: 12
Belongs to series: Molecular and Cellular Endocrinology
ISSN: 0303-7207
DOI: https://doi.org/10.1016/j.mce.2016.03.013
URI: http://hdl.handle.net/10138/223955
Abstract: During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41(iCre/iCre) knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41(iCre/+) mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41(iCre/iCre) sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41(iCre/liCre) sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
Subject: Beta-defensin
Epididymis
Sperm maturation
Flagellar motility pattern
Sperm-oocyte binding
iCre knock-in
TYROSINE PHOSPHORYLATION
HYPERACTIVATED MOTILITY
RAT SPERMATOZOA
MALE-FERTILITY
MOVEMENT CHARACTERISTICS
REPRODUCTIVE-TRACT
GENE CLUSTERS
PROTEIN
CAPACITATION
MICE
1182 Biochemistry, cell and molecular biology
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