Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding

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dc.contributor.author Bjorkgren, Ida
dc.contributor.author Alvarez, Luis
dc.contributor.author Blank, Nelli
dc.contributor.author Balbach, Melanie
dc.contributor.author Turunen, Heikki
dc.contributor.author Laajala, Teemu Daniel
dc.contributor.author Toivanen, Jussi
dc.contributor.author Krutskikh, Anton
dc.contributor.author Wahlberg, Niklas
dc.contributor.author Huhtaniemi, Ilpo
dc.contributor.author Poutanen, Matti
dc.contributor.author Wachten, Dagmar
dc.contributor.author Sipila, Petra
dc.date.accessioned 2017-09-14T09:53:02Z
dc.date.available 2021-12-17T18:48:32Z
dc.date.issued 2016-05-15
dc.identifier.citation Bjorkgren , I , Alvarez , L , Blank , N , Balbach , M , Turunen , H , Laajala , T D , Toivanen , J , Krutskikh , A , Wahlberg , N , Huhtaniemi , I , Poutanen , M , Wachten , D & Sipila , P 2016 , ' Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding ' , Molecular and Cellular Endocrinology , vol. 427 , no. C , pp. 143-154 . https://doi.org/10.1016/j.mce.2016.03.013
dc.identifier.other PURE: 64175621
dc.identifier.other PURE UUID: b6edb5ec-40b6-486c-85ac-317fb566a857
dc.identifier.other WOS: 000375335500015
dc.identifier.other Scopus: 84961226133
dc.identifier.uri http://hdl.handle.net/10138/223955
dc.description.abstract During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41(iCre/iCre) knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41(iCre/+) mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41(iCre/iCre) sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41(iCre/liCre) sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation. (C) 2016 Elsevier Ireland Ltd. All rights reserved. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Molecular and Cellular Endocrinology
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Beta-defensin
dc.subject Epididymis
dc.subject Sperm maturation
dc.subject Flagellar motility pattern
dc.subject Sperm-oocyte binding
dc.subject iCre knock-in
dc.subject TYROSINE PHOSPHORYLATION
dc.subject HYPERACTIVATED MOTILITY
dc.subject RAT SPERMATOZOA
dc.subject MALE-FERTILITY
dc.subject MOVEMENT CHARACTERISTICS
dc.subject REPRODUCTIVE-TRACT
dc.subject GENE CLUSTERS
dc.subject PROTEIN
dc.subject CAPACITATION
dc.subject MICE
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding en
dc.type Article
dc.contributor.organization Institute for Molecular Medicine Finland
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.mce.2016.03.013
dc.relation.issn 0303-7207
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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