dc.contributor.author |
Bjorkgren, Ida |
|
dc.contributor.author |
Alvarez, Luis |
|
dc.contributor.author |
Blank, Nelli |
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dc.contributor.author |
Balbach, Melanie |
|
dc.contributor.author |
Turunen, Heikki |
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dc.contributor.author |
Laajala, Teemu Daniel |
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dc.contributor.author |
Toivanen, Jussi |
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dc.contributor.author |
Krutskikh, Anton |
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dc.contributor.author |
Wahlberg, Niklas |
|
dc.contributor.author |
Huhtaniemi, Ilpo |
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dc.contributor.author |
Poutanen, Matti |
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dc.contributor.author |
Wachten, Dagmar |
|
dc.contributor.author |
Sipila, Petra |
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dc.date.accessioned |
2017-09-14T09:53:02Z |
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dc.date.available |
2021-12-17T18:48:32Z |
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dc.date.issued |
2016-05-15 |
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dc.identifier.citation |
Bjorkgren , I , Alvarez , L , Blank , N , Balbach , M , Turunen , H , Laajala , T D , Toivanen , J , Krutskikh , A , Wahlberg , N , Huhtaniemi , I , Poutanen , M , Wachten , D & Sipila , P 2016 , ' Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding ' , Molecular and Cellular Endocrinology , vol. 427 , no. C , pp. 143-154 . https://doi.org/10.1016/j.mce.2016.03.013 |
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dc.identifier.other |
PURE: 64175621 |
|
dc.identifier.other |
PURE UUID: b6edb5ec-40b6-486c-85ac-317fb566a857 |
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dc.identifier.other |
WOS: 000375335500015 |
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dc.identifier.other |
Scopus: 84961226133 |
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dc.identifier.uri |
http://hdl.handle.net/10138/223955 |
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dc.description.abstract |
During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41(iCre/iCre) knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41(iCre/+) mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41(iCre/iCre) sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41(iCre/liCre) sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation. (C) 2016 Elsevier Ireland Ltd. All rights reserved. |
en |
dc.format.extent |
12 |
|
dc.language.iso |
eng |
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dc.relation.ispartof |
Molecular and Cellular Endocrinology |
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dc.rights.uri |
info:eu-repo/semantics/openAccess |
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dc.subject |
Beta-defensin |
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dc.subject |
Epididymis |
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dc.subject |
Sperm maturation |
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dc.subject |
Flagellar motility pattern |
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dc.subject |
Sperm-oocyte binding |
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dc.subject |
iCre knock-in |
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dc.subject |
TYROSINE PHOSPHORYLATION |
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dc.subject |
HYPERACTIVATED MOTILITY |
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dc.subject |
RAT SPERMATOZOA |
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dc.subject |
MALE-FERTILITY |
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dc.subject |
MOVEMENT CHARACTERISTICS |
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dc.subject |
REPRODUCTIVE-TRACT |
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dc.subject |
GENE CLUSTERS |
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dc.subject |
PROTEIN |
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dc.subject |
CAPACITATION |
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dc.subject |
MICE |
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dc.subject |
1182 Biochemistry, cell and molecular biology |
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dc.title |
Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding |
en |
dc.type |
Article |
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dc.contributor.organization |
Institute for Molecular Medicine Finland |
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dc.description.reviewstatus |
Peer reviewed |
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dc.relation.doi |
https://doi.org/10.1016/j.mce.2016.03.013 |
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dc.relation.issn |
0303-7207 |
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dc.rights.accesslevel |
openAccess |
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dc.type.version |
publishedVersion |
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