A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study

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http://hdl.handle.net/10138/224049

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Itokazu , Y , Tajima , N , Kerosuo , L , Somerharju , P , Sariola , H , Yu , R K & Kakela , R 2016 , ' A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study ' , Neurochemical Research , vol. 41 , no. 7 , pp. 1527-1544 . https://doi.org/10.1007/s11064-016-1867-3

Julkaisun nimi: A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study
Tekijä: Itokazu, Yutaka; Tajima, Nobuyoshi; Kerosuo, Laura; Somerharju, Pentti; Sariola, Hannu; Yu, Robert K.; Kakela, Reijo
Tekijän organisaatio: Medicum
Department of Biochemistry and Developmental Biology
Biosciences
Hannu Sariola / Principal Investigator
Functional Lipidomics Group
Päiväys: 2016-07
Kieli: eng
Sivumäärä: 18
Kuuluu julkaisusarjaan: Neurochemical Research
ISSN: 0364-3190
DOI-tunniste: https://doi.org/10.1007/s11064-016-1867-3
URI: http://hdl.handle.net/10138/224049
Tiivistelmä: The central nervous system (CNS) harbors multiple glial fibrillary acidic protein (GFAP) expressing cell types. In addition to the most abundant cell type of the CNS, the astrocytes, various stem cells and progenitor cells also contain GFAP+ populations. Here, in order to distinguish between two types of GFAP expressing cells with or without the expression of the A2B5 antigens, we performed lipidomic analyses on A2B5+/GFAP+ and A2B5-/GFAP+ cells from rat spinal cord. First, A2B5+/GFAP- progenitors were exposed to the leukemia inhibitory factor (LIF) or bone morphogenetic protein (BMP) to induce their differentiation to A2B5+/GFAP+ cells or A2B5-/GFAP+ astrocytes, respectively. The cells were then analyzed for changes in their phospholipid, sphingolipid or acyl chain profiles by mass spectrometry and gas chromatography. Compared to A2B5+/GFAP- progenitors, A2B5-/GFAP+ astrocytes contained higher amounts of ether phospholipids (especially the species containing arachidonic acid) and sphingomyelin, which may indicate characteristics of cellular differentiation and inability for multipotency. In comparison, principal component analyses revealed that the lipid composition of A2B5+/GFAP+ cells retained many of the characteristics of A2B5+/GFAP- progenitors, but their lipid profile was different from that of A2B5-/GFAP+ astrocytes. Thus, our study demonstrated that two GFAP+ cell populations have distinct lipid profiles with the A2B5+/GFAP+ cells sharing a phospholipid profile with progenitors rather than astrocytes. The progenitor cells may require regulated low levels of lipids known to mediate signaling functions in differentiated cells, and the precursor lipid profiles may serve as one measure of the differentiation capacity of a cell population.
Avainsanat: A2B5 antigen
Astrocyte
Glial fibrillary acidic protein
Glial progenitor/precursor cell
Lipidomics
Mass spectrometry
Phospholipid
Sphingolipid
RESTRICTED PRECURSOR CELLS
WHITE-MATTER
STEM-CELLS
SPECTROMETRIC ANALYSIS
QUANTITATIVE-ANALYSIS
MONOCLONAL-ANTIBODY
MASS-SPECTROMETRY
ARACHIDONIC-ACID
CHAIN LENGTH
FATTY-ACID
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
Vertaisarvioitu: Kyllä
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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