A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study

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dc.contributor.author Itokazu, Yutaka
dc.contributor.author Tajima, Nobuyoshi
dc.contributor.author Kerosuo, Laura
dc.contributor.author Somerharju, Pentti
dc.contributor.author Sariola, Hannu
dc.contributor.author Yu, Robert K.
dc.contributor.author Kakela, Reijo
dc.date.accessioned 2017-09-14T09:55:21Z
dc.date.available 2021-12-17T18:49:30Z
dc.date.issued 2016-07
dc.identifier.citation Itokazu , Y , Tajima , N , Kerosuo , L , Somerharju , P , Sariola , H , Yu , R K & Kakela , R 2016 , ' A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study ' , Neurochemical Research , vol. 41 , no. 7 , pp. 1527-1544 . https://doi.org/10.1007/s11064-016-1867-3
dc.identifier.other PURE: 65750143
dc.identifier.other PURE UUID: c996eb2f-fcb0-46ab-81f5-571148bb2a94
dc.identifier.other WOS: 000377474000003
dc.identifier.other Scopus: 84975763613
dc.identifier.uri http://hdl.handle.net/10138/224049
dc.description.abstract The central nervous system (CNS) harbors multiple glial fibrillary acidic protein (GFAP) expressing cell types. In addition to the most abundant cell type of the CNS, the astrocytes, various stem cells and progenitor cells also contain GFAP+ populations. Here, in order to distinguish between two types of GFAP expressing cells with or without the expression of the A2B5 antigens, we performed lipidomic analyses on A2B5+/GFAP+ and A2B5-/GFAP+ cells from rat spinal cord. First, A2B5+/GFAP- progenitors were exposed to the leukemia inhibitory factor (LIF) or bone morphogenetic protein (BMP) to induce their differentiation to A2B5+/GFAP+ cells or A2B5-/GFAP+ astrocytes, respectively. The cells were then analyzed for changes in their phospholipid, sphingolipid or acyl chain profiles by mass spectrometry and gas chromatography. Compared to A2B5+/GFAP- progenitors, A2B5-/GFAP+ astrocytes contained higher amounts of ether phospholipids (especially the species containing arachidonic acid) and sphingomyelin, which may indicate characteristics of cellular differentiation and inability for multipotency. In comparison, principal component analyses revealed that the lipid composition of A2B5+/GFAP+ cells retained many of the characteristics of A2B5+/GFAP- progenitors, but their lipid profile was different from that of A2B5-/GFAP+ astrocytes. Thus, our study demonstrated that two GFAP+ cell populations have distinct lipid profiles with the A2B5+/GFAP+ cells sharing a phospholipid profile with progenitors rather than astrocytes. The progenitor cells may require regulated low levels of lipids known to mediate signaling functions in differentiated cells, and the precursor lipid profiles may serve as one measure of the differentiation capacity of a cell population. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof Neurochemical Research
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject A2B5 antigen
dc.subject Astrocyte
dc.subject Glial fibrillary acidic protein
dc.subject Glial progenitor/precursor cell
dc.subject Lipidomics
dc.subject Mass spectrometry
dc.subject Phospholipid
dc.subject Sphingolipid
dc.subject WHITE-MATTER
dc.subject STEM-CELLS
dc.subject CHAIN LENGTH
dc.subject FATTY-ACID
dc.subject 3111 Biomedicine
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Department of Biochemistry and Developmental Biology
dc.contributor.organization Biosciences
dc.contributor.organization Hannu Sariola / Principal Investigator
dc.contributor.organization Functional Lipidomics Group
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1007/s11064-016-1867-3
dc.relation.issn 0364-3190
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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