Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro

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Gateva , G , Kremneva , E , Reindl , T , Kotila , T , Kogan , K , Gressin , L , Gunning , P W , Manstein , D J , Michelot , A & Lappalainen , P 2017 , ' Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro ' , Current Biology , vol. 27 , no. 5 , pp. 705-713 . https://doi.org/10.1016/j.cub.2017.01.018

Title: Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro
Author: Gateva, Gergana; Kremneva, Elena; Reindl, Theresia; Kotila, Tommi; Kogan, Konstantin; Gressin, Laurene; Gunning, Peter W.; Manstein, Dietmar J.; Michelot, Alphee; Lappalainen, Pekka
Contributor organization: Institute of Biotechnology
Pekka Lappalainen / Principal Investigator
Date: 2017-03-06
Language: eng
Number of pages: 9
Belongs to series: Current Biology
ISSN: 0960-9822
DOI: https://doi.org/10.1016/j.cub.2017.01.018
URI: http://hdl.handle.net/10138/224239
Abstract: Actin filaments assemble into a variety of networks to provide force for diverse cellular processes [1]. Tropomyosins are coiled-coil dimers that form head-to-tail polymers along actin filaments and regulate interactions of other proteins, including actin-de polymerizing factor (ADF)/cofilins and myosins, with actin [2-5]. In mammals, >40 tropomyosin isoforms can be generated through alternative splicing from four tropomyosin genes. Different isoforms display non-redundant functions and partially non-overlapping localization patterns, for example within the stress fiber network [6, 7]. Based on cell biological studies, it was thus proposed that tropomyosin isoforms may specify the functional properties of different actin filament populations [2]. To test this hypothesis, we analyzed the properties of actin filaments decorated by stress-fiber-associated tropomyosins (Tpm1.6, Tpm1.7, Tpm2.1, Tpm3.1, Tpm3.2, and Tpm4.2). These proteins bound F-actin with high affinity and competed with a-actinin for actin filament binding. Importantly, total internal reflection fluorescence (TIRF) microscopy of fluorescently tagged proteins revealed that most tropomyosin isoforms cannot co-polymerize with each other on actin filaments. These isoforms also bind actin with different dynamics, which correlate with their effects on actin-binding proteins. The long isoforms Tpm1.6 and Tpm1.7 displayed stable interactions with actin filaments and protected filaments from ADF/cofilin-mediated disassembly, but did not activate non-muscle myosin Ila (NMIIa). In contrast, the short isoforms Tpm3.1, Tpm3.2, and Tpm4.2 displayed rapid dynamics on actin filaments and stimulated the ATPase activity of NMIla, but did not efficiently protect filaments from ADF/cofilin. Together, these data provide experimental evidence that tropomyosin isoforms segregate to different actin filaments and specify functional properties of distinct actin filament populations.
Subject: STRESS FIBERS
F-ACTIN
MYOSIN-II
COFILIN
BINDING
YEAST
SPECIFICATION
RECRUITMENT
NETWORKS
MODULATE
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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