Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans

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Mikkonen , E , Haglund , C & Holmberg , C I 2017 , ' Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans ' , PLoS One , vol. 12 , no. 8 , 0183403 . https://doi.org/10.1371/journal.pone.0183403

Title: Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans
Author: Mikkonen, Elisa; Haglund, Caj; Holmberg, Carina I.
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Clinicum
University of Helsinki, Research Programs Unit
Date: 2017-08-17
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/224275
Abstract: The ubiquitin-proteasome system (UPS) plays a crucial part in normal cell function by mediating intracellular protein clearance. We have previously shown that UPS-mediated protein degradation varies in a cell type-specific manner in C. elegans. Here, we use formalin-fixed, paraffin-embedded C. elegans sections to enable studies on endogenous proteasome tissue expression. We show that the proteasome immunoreactivity pattern differs between cell types and within subcellular compartments in adult wild-type (N2) C. elegans. Interestingly, widespread knockdown of proteasome subunits by RNAi results in tissue-specific changes in proteasome expression instead of a uniform response. In addition, long-lived daf-2 (e1370) mutants with impaired insulin/IGF-1 signaling (IIS) display similar proteasome tissue expression as aged-matched wild-type animals. Our study emphasizes the importance of alternate approaches to the commonly used whole animal lysate-based methods to detect changes in proteasome expression occurring at the sub-cellular, cell or tissue resolution level in a multicellular organism.
Subject: CAENORHABDITIS-ELEGANS
TRANSCRIPTION FACTOR
26S PROTEASOME
INHIBITION
STRESS
FIBROBLASTS
DEGRADATION
ACTIVATION
LONGEVITY
TURNOVER
3122 Cancers
3126 Surgery, anesthesiology, intensive care, radiology
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