Cellular delivery of enzyme-loaded DNA origami

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http://hdl.handle.net/10138/224383

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Ora , A , Järvihaavisto , E , Zhang , H , Auvinen , H , Santos , H A , Kostiainen , M A & Linko , V 2016 , ' Cellular delivery of enzyme-loaded DNA origami ' , Chemical Communications , vol. 52 , no. 98 , pp. 14161-14164 . https://doi.org/10.1039/c6cc08197e

Title: Cellular delivery of enzyme-loaded DNA origami
Author: Ora, Ari; Järvihaavisto, Erika; Zhang, Hongbo; Auvinen, Henni; Santos, Helder A.; Kostiainen, Mauri A.; Linko, Veikko
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2016
Language: eng
Number of pages: 4
Belongs to series: Chemical Communications
ISSN: 1359-7345
URI: http://hdl.handle.net/10138/224383
Abstract: In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a compelling candidate for a great variety of delivery applications.
Subject: INTRACELLULAR PROTEIN DELIVERY
DRUG-DELIVERY
NANOSCALE SHAPES
CANCER-THERAPY
FOLDING DNA
NANOSTRUCTURES
NANOPARTICLES
NANOCARRIERS
RESISTANCE
NANOTECHNOLOGY
116 Chemical sciences
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