Virus found in a boreal lake links ssDNA and dsDNA viruses

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Laanto , E , Mantynen , S , De Colibus , L , Marjakangas , J , Gillum , A , Stuart , D I , Ravantti , J J , Huiskonen , J T & Sundberg , L-R 2017 , ' Virus found in a boreal lake links ssDNA and dsDNA viruses ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 114 , no. 31 , pp. 8378-8383 . https://doi.org/10.1073/pnas.1703834114

Title: Virus found in a boreal lake links ssDNA and dsDNA viruses
Author: Laanto, Elina; Mantynen, Sari; De Colibus, Luigi; Marjakangas, Jenni; Gillum, Ashley; Stuart, David I.; Ravantti, Janne J.; Huiskonen, Juha T.; Sundberg, Lotta-Riina
Contributor: University of Helsinki, University of Jyväskylä
University of Helsinki, Research Programs Unit
University of Helsinki, Biosciences
Date: 2017-08-01
Language: eng
Number of pages: 6
Belongs to series: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
URI: http://hdl.handle.net/10138/224402
Abstract: Viruses have impacted the biosphere in numerous ways since the dawn of life. However, the evolution, genetic, structural, and taxonomic diversity of viruses remain poorly understood, in part because sparse sampling of the virosphere has concentrated mostly on exploring the abundance and diversity of dsDNA viruses. Furthermore, viral genomes are highly diverse, and using only the current sequence-based methods for classifying viruses and studying their phylogeny is complicated. Here we describe a virus, FLiP (Flavobacterium-infecting, lipid-containing phage), with a circular ssDNA genome and an internal lipid membrane enclosed in the icosahedral capsid. The 9,174-nt-long genome showed limited sequence similarity to other known viruses. The genetic data imply that this virus might use replication mechanisms similar to those found in other ssDNA replicons. However, the structure of the viral major capsid protein, elucidated at near-atomic resolution using cryo-electron microscopy, is strikingly similar to that observed in dsDNA viruses of the PRD1-adenovirus lineage, characterized by a major capsid protein bearing two beta-barrels. The strong similarity between FLiP and another member of the structural lineage, bacteriophage PM2, extends to the capsid organization (pseudo T = 21 dextro) despite the difference in the genetic material packaged and the lack of significant sequence similarity.
Subject: cryo-electron microscopy
Flavobacterium
lipids
genome
structure
CRYOELECTRON MICROSCOPY
PROTEIN STRUCTURES
VIRAL UNIVERSE
CAPSID PROTEIN
DNA
EVOLUTION
BACTERIAL
GENOMES
BINDING
COMMUNITIES
1183 Plant biology, microbiology, virology
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