Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models

Show full item record



Permalink

http://hdl.handle.net/10138/224481

Citation

Al-Samadi , A , Awad , S A , Tuomainen , K , Zhao , Y , Salem , A , Parikka , M & Salo , T 2017 , ' Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models ' , Oncotarget , vol. 8 , no. 36 , pp. 60123-60134 . https://doi.org/10.18632/oncotarget.17768

Title: Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models
Author: Al-Samadi, Ahmed; Awad, Shady Adnan; Tuomainen, Katja; Zhao, Yue; Salem, Abdelhakim; Parikka, Mataleena; Salo, Tuula
Contributor: University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2017-09-01
Language: eng
Number of pages: 12
Belongs to series: Oncotarget
ISSN: 1949-2553
URI: http://hdl.handle.net/10138/224481
Abstract: The crosstalk between immune cells, cancer cells, and extracellular vesicles (EVs) secreted by cancer cells remains poorly understood. We created three-dimensional (3D) cell culture models using human leiomyoma discs and Myogel to study the effects of immune cells on highly (HSC-3) and less (SCC-25) invasive oral tongue squamous cell carcinoma (OTSCC) cell lines. Additionally, we studied the effects of EVs isolated from these cell lines on the cytotoxicity of CD8(+) T and NK cells isolated from three healthy donors. Our analysis included the effects of these EVs on innate immunity in zebrafish larvae. Activated immune cells significantly decreased the proliferation of both OTSCC cell lines and associated with a diminished invasion area of HSC-3 cells. In general, EVs from SCC-25 increased the cytotoxic activity of CD8(+) T and NK cells more than those from HSC-3 cells. However, this effect varied depending on the source and the immune and cancer cell subgroups. In zebrafish, the amount of IL-13 mRNA was decreased by SCC-25 EVs. This study describes promising in vitro and in vivo models to investigate interactions between immune cells, cancer cells, and EVs.
Subject: tongue cancer
immune cells
extracellular vesicles
in vitro model
cytotoxicity
MESENCHYMAL STEM-CELLS
TUMOR MICROENVIRONMENT
CANCER-CELLS
PERIPHERAL-BLOOD
EXOSOMES
INFLAMMATION
ZEBRAFISH
INVASION
PROLIFERATION
MACROPHAGES
3122 Cancers
313 Dentistry
3125 Otorhinolaryngology, ophthalmology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
17768_259577_4_PB_1.pdf 6.837Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record