Diazoxide Attenuates Ischemic Myocardial Injury in a Porcine Model

Show full item record



Permalink

http://hdl.handle.net/10138/224541

Citation

Sarja , H , Anttila , T , Mustonen , C , Honkanen , H-P , Herajarvi , J , Haapanen , H , Tuominen , H , Miinalainen , I , Juvonen , T & Anttila , V 2017 , ' Diazoxide Attenuates Ischemic Myocardial Injury in a Porcine Model ' , Heart Surgery Forum , vol. 20 , no. 4 , pp. E153-E161 . https://doi.org/10.1532/hsf.1790

Title: Diazoxide Attenuates Ischemic Myocardial Injury in a Porcine Model
Author: Sarja, Henna; Anttila, Tuomas; Mustonen, Caius; Honkanen, Hannu-Pekka; Herajarvi, Johanna; Haapanen, Henri; Tuominen, Hannu; Miinalainen, Ilkka; Juvonen, Tatu; Anttila, Vesa
Contributor: University of Helsinki, Clinicum
Date: 2017
Language: eng
Number of pages: 9
Belongs to series: Heart Surgery Forum
ISSN: 1098-3511
URI: http://hdl.handle.net/10138/224541
Abstract: Background: We hypothesized that diazoxide, a mitochondrial ATP-sensitive potassium channel opener, has cardioprotective effects during acute myocardial ischemia. Diazoxide is suggested to act through protein kinase Ce (PKC epsilon) activation. Methods: Twelve piglets were randomly assigned to receive intravenous infusion of diazoxide (3.5 mg/kg) with solvent or only solvent (6 animals per group) before cardiac ischemia. Myocardial ischemia was induced by occluding the left circumflex artery (LCX) for 40 minutes. The reperfusion and follow-up period lasted for three hours. Throughout the experiment hemodynamic measurements and blood samples were collected, and after the follow-up period the hearts were harvested for transmission electron microscopy (TEM) as well as histopathological and immunohistochemical analyses. Results: TEM showed less ischemic damage on a cellular level in the diazoxide group (P = .004) than in the control group. Creatinine kinase MB levels (Pt*g = .030) were lower, and oxygen consumption (Pt*g = .037) and delivery (Pg = .038) were higher in the diazoxide group compared to the controls. Conclusion: Diazoxide preserves myocardial cellular structure and cellular function, and thus it may have benefits in treating ischemic myocardial injury.
Subject: K-ATP CHANNELS
KINASE-C-EPSILON
CARDIAC MITOCHONDRIA
POSSIBLE MECHANISM
OXIDATIVE STRESS
CA2+ OVERLOAD
CARDIOPROTECTION
REPERFUSION
HEART
CARDIOPLEGIA
3121 General medicine, internal medicine and other clinical medicine
3126 Surgery, anesthesiology, intensive care, radiology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Diazoxide_Attenuates_Ischemic.pdf 1.834Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record