Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension

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Tachikawa , M , Morone , N , Senju , Y , Sugiura , T , Hanawa-Suetsugu , K , Mochizuki , A & Suetsugu , S 2017 , ' Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension ' , Scientific Reports , vol. 7 , 7794 . https://doi.org/10.1038/s41598-017-08259-5

Title: Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
Author: Tachikawa, Masashi; Morone, Nobuhiro; Senju, Yosuke; Sugiura, Tadao; Hanawa-Suetsugu, Kyoko; Mochizuki, Atsushi; Suetsugu, Shiro
Contributor: University of Helsinki, Institute of Biotechnology
Date: 2017-08-10
Language: eng
Number of pages: 14
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/224547
Abstract: Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.
Subject: ELECTRON-MICROSCOPY
PLASMA-MEMBRANE
PROTEIN
DISTANCE
1182 Biochemistry, cell and molecular biology
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