Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes

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http://hdl.handle.net/10138/224612

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Oliinyk , O S , Chernov , K G & Verkhusha , V V 2017 , ' Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes ' , International Journal of Molecular Sciences , vol. 18 , no. 8 , 1691 . https://doi.org/10.3390/ijms18081691

Title: Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes
Author: Oliinyk, Olena S.; Chernov, Konstantin G.; Verkhusha, Vladislav V.
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
Date: 2017-08
Language: eng
Number of pages: 27
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
URI: http://hdl.handle.net/10138/224612
Abstract: Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors. We next analyze molecular evolution approaches to develop NIR FPs and biosensors. We then discuss phenotypes of current BphP-based NIR FPs and compare them with FPs derived from CBCRs and APCs. Lastly, we overview imaging applications of NIR FPs in live cells and in vivo. Our review provides guidelines for selection of existing NIR FPs, as well as engineering approaches to develop NIR FPs from the novel natural templates such as CBCRs.
Subject: bacterial photoreceptor
near-infrared fluorescent protein
phytochrome
cyanobacteriochrome
allophycocyanin
tetrapyrrole
COMPLEMENTARY CHROMATIC ADAPTATION
HUMAN BILIVERDIN REDUCTASE
EXCITED-STATE DYNAMICS
FAR-RED LIGHT
IN-VIVO
RED/GREEN CYANOBACTERIOCHROME
ESCHERICHIA-COLI
GAF DOMAIN
CRYSTAL-STRUCTURE
SYNECHOCYSTIS SP
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
116 Chemical sciences
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