Surface bioengineering of diatomite based nanovectors for efficient intracellular uptake and drug delivery

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http://hdl.handle.net/10138/224647

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Terracciano , M , Shahbazi , M-A , Correia , A , Rea , I , Lamberti , A , De Stefano , L & Santos , H A 2015 , ' Surface bioengineering of diatomite based nanovectors for efficient intracellular uptake and drug delivery ' , Nanoscale , vol. 7 , no. 47 , pp. 20063-20074 . https://doi.org/10.1039/c5nr05173h

Title: Surface bioengineering of diatomite based nanovectors for efficient intracellular uptake and drug delivery
Author: Terracciano, Monica; Shahbazi, Mohammad-Ali; Correia, Alexandra; Rea, Ilaria; Lamberti, Annalisa; De Stefano, Luca; Santos, Hélder A.
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2015
Language: eng
Number of pages: 12
Belongs to series: Nanoscale
ISSN: 2040-3364
URI: http://hdl.handle.net/10138/224647
Abstract: Diatomite is a natural porous silica material of sedimentary origin. Due to its peculiar properties, it can be considered as a valid surrogate of synthetic porous silica for nano-based drug delivery. In this work, we exploit the potential of diatomite nanoparticles (DNPs) for drug delivery with the aim of developing a successful dual-biofunctionalization method by polyethylene glycol (PEG) coverage and cell-penetrating peptide (CPP) bioconjugation, to improve the physicochemical and biological properties of the particles, to enhance the intracellular uptake in cancer cells, and to increase the biocompatibility of 3-aminopropyltriethoxysilane (APT) modified-DNPs. DNPs-APT-PEG-CPP showed hemocompatibility for up to 200 mu g mL(-1) after 48 h of incubation with erythrocytes, with a hemolysis value of only 1.3%. The cytotoxicity of the modified-DNPs with a concentration up to 200 mu g mL(-1) and incubation with MCF-7 and MDA-MB-231 breast cancer cells for 24 h, demonstrated that PEGylation and CPP-bioconjugation can strongly reduce the cytotoxicity of DNPs-APT. The cellular uptake of the modified-DNPs was also evaluated using the above mentioned cancer cell lines, showing that the CPP-bioconjugation can considerably increase the DNP cellular uptake. Moreover, the dual surface modification of DNPs improved both the loading of a poorly water-soluble anticancer drug, sorafenib, with a loading degree up to 22 wt%, and also enhanced the drug release profiles in aqueous solutions. Overall, this work demonstrates that the biofunctionalization of DNPs is a promising platform for drug delivery applications in cancer therapy as a result of its enhanced stability, biocompatibility, cellular uptake, and drug release profiles.
Subject: CELL-PENETRATING PEPTIDES
POROUS SILICON NANOPARTICLES
IN-VITRO CYTOTOXICITY
CANCER-TREATMENT
NANOTECHNOLOGY
RELEASE
MICROPARTICLES
NANOMEDICINES
FABRICATION
CHALLENGES
317 Pharmacy
221 Nano-technology
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