Protein profiling of nasopharyngeal aspirates of hospitalized and outpatients revealed cytokines associated with severe influenza A(H1N1)pdm09 virus infections: A pilot study

Show full item record



Permalink

http://hdl.handle.net/10138/225037

Citation

Fu , Y , Gaelings , L , Jalovaara , P , Kakkola , L , Kinnunen , M T , Kallio-Kokko , H , Valkonen , M , Kantele , A & Kainov , D E 2016 , ' Protein profiling of nasopharyngeal aspirates of hospitalized and outpatients revealed cytokines associated with severe influenza A(H1N1)pdm09 virus infections: A pilot study ' , Cytokine , vol. 86 , pp. 10-14 . https://doi.org/10.1016/j.cyto.2016.07.003

Title: Protein profiling of nasopharyngeal aspirates of hospitalized and outpatients revealed cytokines associated with severe influenza A(H1N1)pdm09 virus infections: A pilot study
Author: Fu, Yu; Gaelings, Lana; Jalovaara, Petri; Kakkola, Laura; Kinnunen, Mervi T.; Kallio-Kokko, Hannimari; Valkonen, Miia; Kantele, Anu; Kainov, Denis E.
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Department of Virology
University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Institute for Molecular Medicine Finland
Date: 2016-10
Language: eng
Number of pages: 5
Belongs to series: Cytokine
ISSN: 1043-4666
URI: http://hdl.handle.net/10138/225037
Abstract: Influenza A viruses (IAV) mutate rapidly and cause seasonal epidemics and occasional pandemics, which result in substantial number of patient visits to the doctors and even hospitalizations. We aimed here to identify inflammatory proteins, which levels correlated to clinical severity of the disease. For this we analysed 102 cytokines and growth factors in human nasopharyngeal aspirate (NPA) samples of 27 hospitalized and 27 outpatients diagnosed with influenza A(H1N1)pdm09 virus infection. We found that the relative levels of monocyte differentiation antigen CD14, lipocalin-2 (LCN2), C-C-motif chemokine 20 (CCL20), CD147, urokinase plasminogen activator surface receptor (uPAR), pro-epidermal growth factor (EGF), trefoil factor 3 (TFF3), and macrophage migration inhibitory factor (MIF) were significantly lower (p <0.008), whereas levels of retinol-binding protein 4 (RBP4), C-X-C motif chemokine 5 (CXCL5), interleukin-8 (IL-8), complement factor D (CFD), adiponectin, and chitinase-3-like 1 (CHI3L1) were significantly higher (p <0.008) in NPA samples of hospitalized than non-hospitalized patients. While changes in CD14, LCN2, CCL20, uPAR, EGF, MIF, CXCL5, IL-8, adiponectin and CHI3L1 levels have already been correlated with severity of IAV infection in mice and humans, our study is the first to describe association of CD147, RBP4, TFF3, and CFD with hospitalization of IAV-infected patients. Thus, we identified local innate immune profiles, which were associated with the clinical severity of influenza infections. (C) 2016 Elsevier Ltd. All rights reserved.
Subject: Influenza virus
Cytokine
Chemokine
Nasopharyngeal aspirate
RETINOL-BINDING-PROTEIN
INNATE IMMUNE-RESPONSE
MICE
EXPRESSION
PNEUMONIA
DISEASE
GENE
SUSCEPTIBILITY
VARIABILITY
REGULATOR
1182 Biochemistry, cell and molecular biology
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Protein_profiling_of_nasopharyngeal.pdf 700.7Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record