Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail

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Leskinen , K , Tuomala , H , Wicklund , A , Horsma-Heikkinen , J , Kuusela , P , Skurnik , M & Kiljunen , S 2017 , ' Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail ' , Viruses-Basel , vol. 9 , no. 9 , 258 . https://doi.org/10.3390/v9090258

Title: Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail
Author: Leskinen, Katarzyna; Tuomala, Henni; Wicklund, Anu; Horsma-Heikkinen, Jenni; Kuusela, Pentti; Skurnik, Mikael; Kiljunen, Saija
Other contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Research Programs Unit
University of Helsinki, HUSLAB
University of Helsinki, Research Programs Unit
University of Helsinki, Immunobiology Research Program








Date: 2017-09
Language: eng
Number of pages: 19
Belongs to series: Viruses-Basel
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v9090258
URI: http://hdl.handle.net/10138/225789
Abstract: Staphylococcus aureus is a commensal and pathogenic bacterium that causes infections in humans and animals. It is a major cause of nosocomial infections worldwide. Due to increasing prevalence of multidrug resistance, alternative methods to eradicate the pathogen are necessary. In this respect, polyvalent staphylococcal myoviruses have been demonstrated to be excellent candidates for phage therapy. Here we present the characterization of the bacteriophage vB_SauM-fRuSau02 (fRuSau02) that was isolated from a commercial Staphylococcus bacteriophage cocktail produced by Microgen (Moscow, Russia). The genomic analysis revealed that fRuSau02 is very closely related to the phage MSA6, and possesses a large genome (148,464 bp), with typical modular organization and a low G+ C (30.22%) content. It can therefore be classified as a new virus among the genus Twortlikevirus. The genome contains 236 predicted genes, 4 of which were interrupted by insertion sequences. Altogether, 78 different structural and virion-associated proteins were identified from purified phage particles by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The host range of fRuSau02 was tested with 135 strains, including 51 and 54 Staphylococcus aureus isolates from humans and pigs, respectively, and 30 coagulase-negative Staphylococcus strains of human origin. All clinical S. aureus strains were at least moderately sensitive to the phage, while only 39% of the pig strains were infected. Also, some strains of Staphylococcus intermedius, Staphylococcus lugdunensis, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus and Staphylococcus pseudointer were sensitive. We conclude that fRuSau02, a phage therapy agent in Russia, can serve as an alternative to antibiotic therapy against S. aureus.
Subject: Staphylococcus aureus
bacteriophage
phage therapy
vB_SauM-fRuSau02
Twortlikevirus
STAPHYLOCOCCUS-AUREUS INFECTIONS
INTEGRATIVE GENOMICS VIEWER
GRAM-POSITIVE BACTERIA
FOOD-INDUSTRY
HOST-RANGE
IDENTIFICATION
VISUALIZATION
CONTRIBUTES
EXPLORATION
DEFINITION
3111 Biomedicine
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