Identification of urinary tract pathogens after 3-hours urine culture by MALDI-TOF mass spectrometry

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Haiko , J , Savolainen , L E , Hilla , R & Patari-Sampo , A 2016 , ' Identification of urinary tract pathogens after 3-hours urine culture by MALDI-TOF mass spectrometry ' , Journal of Microbiological Methods , vol. 129 , pp. 81-84 . https://doi.org/10.1016/j.mimet.2016.08.006

Title: Identification of urinary tract pathogens after 3-hours urine culture by MALDI-TOF mass spectrometry
Author: Haiko, Johanna; Savolainen, Laura E.; Hilla, Risto; Patari-Sampo, Anu
Other contributor: University of Helsinki, Medicum
University of Helsinki, Department of Bacteriology and Immunology
University of Helsinki, Medicum



Date: 2016-10
Language: eng
Number of pages: 4
Belongs to series: Journal of Microbiological Methods
ISSN: 0167-7012
DOI: https://doi.org/10.1016/j.mimet.2016.08.006
URI: http://hdl.handle.net/10138/225927
Abstract: Complicated urinary tract infections, such as pyelonephritis, may lead to sepsis. Rapid diagnosis is needed to identify the causative urinary pathogen and to verify the appropriate empirical antimicrobial therapy. We describe here a rapid identification method for urinary pathogens: urine is incubated on chocolate agar for 3 h at 35 degrees C with 5%CO2 and subjected to MALDI-TOF MS analysis by VITEK MS. Overall 207 screened clinical urine samples were tested in parallel with conventional urine culture. The method, called U-si-MALDI-TOF (urine short incubation MALDI-TOF), showed correct identification for 86% of Gram-negative urinary tract pathogens (Escherichia coli, Klebsiella pneumoniae, and other Enterobacteriaceae), when present at >10(5) cfu/ml in culture (n = 107), compared with conventional culture method. However, Gram-positive bacteria (n = 28) were not successfully identified by U-si-MALDI-TOF. This method is especially suitable for rapid identification of E. coli, the most common cause of urinary tract infections and urosepsis. Turnaround time for identification using U-si-MALDI-TOF compared with conventional urine culture was improved from 24 h to 4-6 h. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Urinary tract infection
Rapid identification
Urine culture
MALDI-TOF
DESORPTION IONIZATION-TIME
POSITIVE BLOOD CULTURES
SAMPLES
BACTERIA
SUSCEPTIBILITY
INFECTIONS
MS
1183 Plant biology, microbiology, virology
3111 Biomedicine
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