Extracellular ATP protects endothelial cells against DNA damage

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http://hdl.handle.net/10138/228253

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Aho , J , Helenius , M , Vattulainen-Collanus , S , Alastalo , T-P & Koskenvuo , J 2016 , ' Extracellular ATP protects endothelial cells against DNA damage ' , Purinergic signalling , vol. 12 , no. 3 , pp. 575-581 . https://doi.org/10.1007/s11302-016-9508-5

Title: Extracellular ATP protects endothelial cells against DNA damage
Author: Aho, Joonas; Helenius, Mikko; Vattulainen-Collanus, Sanna; Alastalo, Tero-Pekka; Koskenvuo, Juha
Contributor: University of Helsinki, Lastentautien yksikkö
University of Helsinki, Children's Hospital
University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2016-09
Language: eng
Number of pages: 7
Belongs to series: Purinergic signalling
ISSN: 1573-9538
URI: http://hdl.handle.net/10138/228253
Abstract: Cell damage can lead to rapid release of ATP to extracellular space resulting in dramatic change in local ATP concentration. Evolutionary, this has been considered as a danger signal leading to adaptive responses in adjacent cells. Our aim was to demonstrate that elevated extracellular ATP or inhibition of ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1/CD39) activity could be used to increase tolerance against DNA-damaging conditions. Human endothelial cells, with increased extracellular ATP concentration in cell proximity, were more resistant to irradiation or chemically induced DNA damage evaluated with the DNA damage markers gamma H2AX and phosphorylated p53. In our rat models of DNA damage, inhibiting CD39-driven ATP hydrolysis with POM-1 protected the heart and lung tissues against chemically induced DNA damage. Interestingly, the phenomenon could not be replicated in cancer cells. Our results show that transient increase in extracellular ATP can promote resistance to DNA damage.
Subject: DNA damage
ATP
NTPDase1
CD39
Cancer
Endothelial cell
DOUBLE-STRAND BREAKS
GROWTH-FACTOR RECEPTOR
CANCER-CELLS
IN-VIVO
REPAIR
ACTIVATION
ADENOSINE
PATHWAY
MONOCROTALINE
PYROPHOSPHATE
3112 Neurosciences
3124 Neurology and psychiatry
1182 Biochemistry, cell and molecular biology
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