Viral haemorrhagic septicaemia virus (VHSV Id) infections are detected more consistently using syndromic vs. active surveillance

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http://hdl.handle.net/10138/228261

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Vennerström , P , Välimäki , E , Lyytikäinen , T , Hautaniemi , M , Vidgren , G , Koski , P & Virtala , A-M 2017 , ' Viral haemorrhagic septicaemia virus (VHSV Id) infections are detected more consistently using syndromic vs. active surveillance ' , Diseases of Aquatic Organisms , vol. 126 , no. 2 , pp. 111-123 . https://doi.org/10.3354/dao03161

Title: Viral haemorrhagic septicaemia virus (VHSV Id) infections are detected more consistently using syndromic vs. active surveillance
Author: Vennerström, Pia; Välimäki, Elina; Lyytikäinen, Tapani; Hautaniemi, Maria; Vidgren, Gabriele; Koski, Perttu; Virtala, Anna-Maija
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
Date: 2017-10-18
Language: eng
Number of pages: 13
Belongs to series: Diseases of Aquatic Organisms
ISSN: 0177-5103
URI: http://hdl.handle.net/10138/228261
Abstract: The eradication of viral haemorrhagic septicaemia virus (VHSV Id) from Finnish brackish-water rainbow trout Oncorhynchus mykiss farms located in the restriction zone in the Province of angstrom land, Baltic Sea, failed several times in the 2000s. The official surveillance programme was often unable to find VHSV-positive populations, leading to the misbelief in the fish farming industry that virus eradication could be achieved. The ability of 3 other surveillance programmes to detect infected fish populations was compared with the official programme. One programme involved syndromic surveillance based on the observation of clinical disease signs by fish farmers, while 2 programmes comprised active surveillance similar to the official programme, but included increased sampling frequencies and 2 additional tests. The syndromic surveillance concentrated on sending in samples for analysis when any sign of a possible infectious disease at water temperatures below 15 degrees C was noticed. This programme clearly outperformed active surveillance. A real-time reverse transcriptase-polymerase chain reaction method proved to be at least as sensitive as virus isolation in cell culture in detecting acute VHSV infections. An ELISA method was used to test fish serum for antibodies against VHSV. The ELISA method may be a useful tool in VHSV eradication for screening populations during the follow-up period, before declaring an area free of infection.
Subject: Rainbow trout
Oncorhynchus mykiss
Novirhabdovirus
Rhabdoviridae
Virus eradication
Fish farm
Aquaculture
RT-PCR
ELISA
Finland
LINKED-IMMUNOSORBENT-ASSAY
NORTH-AMERICAN STRAIN
RAINBOW-TROUT
EGTVED VIRUS
LAKE-ONTARIO
MARINE FISH
GENOTYPE
IVB
ANTIBODIES
413 Veterinary science
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