Concurrent estimation of metabolite concentrations along with parent drug quantification in post-mortem blood

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http://hdl.handle.net/10138/228778

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Viinamaki , J & Ojanpera , I 2016 , ' Concurrent estimation of metabolite concentrations along with parent drug quantification in post-mortem blood ' , Forensic Science International , vol. 267 , pp. 110-114 . https://doi.org/10.1016/j.forsciint.2016.08.031

Title: Concurrent estimation of metabolite concentrations along with parent drug quantification in post-mortem blood
Author: Viinamaki, Jenni; Ojanpera, Ilkka
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
Date: 2016-10
Language: eng
Number of pages: 5
Belongs to series: Forensic Science International
ISSN: 0379-0738
URI: http://hdl.handle.net/10138/228778
Abstract: There is a constant demand for the quantification of drug metabolites within post-mortem toxicology. Especially electrospray ionization-mass spectrometry techniques necessitate that calibration is carried out using primary reference standards due to the non-uniform ionization efficiency between parent drugs and their metabolites. As reference standards for metabolites are not readily available and their costs are high, alternative methods for immediate quantification are required. In this study, ultra-high performance liquid chromatography coupled with photodiode array detection and corona charged aerosol detection was utilized for the concurrent quantification of 23 drug metabolites using the corresponding parent drug for calibration. Based on this secondary calibration, the quantitative results for the N-demethylated metabolites by each detector were similar to those obtained by the ordinary calibration using reference standards. For O-demethylated metabolites, the differences in detector response caused somewhat larger biases using the secondary calibration. Using the validated secondary calibration, the blood sample data gathered from 633 post-mortem cases was retrospectively reprocessed to discover the combined metabolite-parent concentrations and metabolite to parent ratios for six toxicologically relevant drugs. These results, representing all causes of death, were compared to published data from therapeutic drug monitoring and post-mortem toxicology. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
Subject: Drug metabolite quantification
Universal detection
Corona charged aerosol detection
Photodiode array detection
Ultra-high performance liquid
chromatography
Post-mortem blood
SINGLE-CALIBRANT QUANTIFICATION
LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY
CHEMILUMINESCENCE NITROGEN DETECTION
EVAPORATIVE LIGHT-SCATTERING
CONSENSUS GUIDELINES
MASS-SPECTROMETRY
VENLAFAXINE
GENOTYPE
DETECTOR
PLASMA
319 Forensic science and other medical sciences
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