Organotypic three-dimensional assays based on human leiomyoma-derived matrices

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Salo , T , Dourado , M R , Sundquist , E , Apu , E H , Alahuhta , I , Tuomainen , K , Vasara , J & Al-Samadi , A 2018 , ' Organotypic three-dimensional assays based on human leiomyoma-derived matrices ' , Philosophical Transactions of the Royal Society. Biological Sciences , vol. 372 , no. 1737 , 20160482 . https://doi.org/10.1098/rstb.2016.0482

Title: Organotypic three-dimensional assays based on human leiomyoma-derived matrices
Author: Salo, Tuula; Dourado, Mauricio Rocha; Sundquist, Elias; Apu, Ehsanul Hoque; Alahuhta, Ilkka; Tuomainen, Katja; Vasara, Jenni; Al-Samadi, Ahmed
Contributor organization: Clinicum
Department of Oral and Maxillofacial Diseases
University of Helsinki
HUS Head and Neck Center
Date: 2018-01-05
Language: eng
Number of pages: 11
Belongs to series: Philosophical Transactions of the Royal Society. Biological Sciences
ISSN: 0962-8436
DOI: https://doi.org/10.1098/rstb.2016.0482
URI: http://hdl.handle.net/10138/229257
Abstract: Alongside cancer cells, tumours exhibit a complex stroma containing a repertoire of cells, matrix molecules and soluble factors that actively crosstalk between each other. Recognition of this multifaceted concept of the tumour microenvironment (TME) calls for authentic TME mimetics to study cancer in vitro. Traditionally, tumourigenesis has been investigated in non-human, three-dimensional rat type I collagen containing organotypic discs or by means of mouse sarcoma-derived gel, such as Matrigel (R). However, the molecular compositions of these simplified assays do not properly simulate human TME. Here, we review the main properties and benefits of using human leiomyoma discs and their matrix Myogel for in vitro assays. Myoma discs are practical for investigating the invasion of cancer cells, as are cocultures of cancer and stromal cells in a stiff, hypoxic TME mimetic. Myoma discs contain soluble factors and matrix molecules commonly present in neoplastic stroma. In Transwell, IncuCyte, spheroid and sandwich assays, cancer cells move faster and form larger colonies in Myogel than in Matrigel (R). Additionally, Myogel can replace Matrigel (R) in hanging-drop and tube-formation assays. Myogel also suits three-dimensional drug testing and extracellular vesicle interactions. To conclude, we describe the application of our myoma-derived matrices in 3D in vitro cancer assays. This article is part of the discussion meeting issue 'Extracellular vesicles and the tumour microenvironment'.
Subject: in vitro cancer invasion
3D
drug testing
CANCER-ASSOCIATED FIBROBLASTS
EPITHELIAL-MESENCHYMAL TRANSITION
SQUAMOUS-CELL CARCINOMA
TUMOR MICROENVIRONMENT
IN-VITRO
BREAST-CANCER
TONGUE CANCER
EXTRACELLULAR VESICLES
COLORECTAL-CANCER
ENDOTHELIAL-CELLS
3122 Cancers
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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