Bradykinin -induced vasodilatation : Role of age, ACE1-inhibitory peptide, mas- and bradykinin receptors

Show simple item record Siltari, Aino Korpela, R. Vapaatalo, H. 2017-12-08T22:07:25Z 2021-12-17T18:45:59Z 2016-11
dc.identifier.citation Siltari , A , Korpela , R & Vapaatalo , H 2016 , ' Bradykinin -induced vasodilatation : Role of age, ACE1-inhibitory peptide, mas- and bradykinin receptors ' , Peptides , vol. 85 , pp. 46-55 .
dc.identifier.other PURE: 72571545
dc.identifier.other PURE UUID: 8df78866-e1db-4bfb-acf3-148642fa9bb4
dc.identifier.other WOS: 000387299200005
dc.identifier.other Scopus: 84987846225
dc.identifier.other ORCID: /0000-0002-7814-427X/work/39321062
dc.identifier.other ORCID: /0000-0003-0337-9186/work/53183927
dc.description.abstract Bradykinin exerts its vascular actions via two types of receptors, the non-constitutively expressed bradykinin receptor type 1 (BR1) and the constitutive type 2 receptor (BR2). Bradykinin-induced vasorelaxation is age-dependent, a phenomenon related to the varying amounts of BR1 and BR2 in the vasculature. Isoleucine-proline-proline (Ile-Pro-Pro), a bioactive tripeptide, lowers elevated blood pressure and improves impaired endothelium-dependent vasorelaxation in hypertensive rats. It inhibits angiotensin converting enzyme 1 (ACE1). Other mechanisms of action have also been postulated. The aims of the study were to clarify the underlying mechanisms of the age-dependency of bradykinin-induced vasodilatation such as the roles of the two bradykinin receptors, themas-receptor and synergism with Ile-Pro-Pro. The vascular response studies were conducted using mesenteric artery and aorta rings from normotensive 6 wk. (young) and 22 wk. (old) Wistar rats. Cumulative dosing of acetylcholine, bradykinin and angiotensin(1-7) (Ang(1-7))were tested in phenylephrine-induced vasoconstriction with or without 10 min pre-incubation with antagonists against BR1-, BR2- or mas-receptors,Ang(1-7) or ACE1-inhibitors captopril and Ile-Pro-Pro. The bradykinin-induced vasorelaxation in vitro was age-dependent and it was improved by pre incubation with Ile-Pro-Pro, especially in old rats with endothelial dysfunction. The mas-receptor antagonist, D-Pro7-Ang(1-7) abolished bradykinin-induced relaxation totally. Interestingly, BR1 and BR2 antagonists only slightly reduced bradykinin-induced vasorelaxation, as an evidence for the involvement of other mechanisms in addition to receptor activation. In conclusion, bradykinin-induced vasorelaxation was age -dependent and He-Pro-Pro improved it. Mas receptor antagonist abolished relaxation while bradykinin receptor antagonist only slightly reduced it, suggesting that bradykinin-induced vasorelaxation is regulated also by other mechanisms than the classical BR1/BR2 pathway. (C) 2016 Elsevier Inc: All rights reserved. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Peptides
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Bradykinin
dc.subject Vasodilatation
dc.subject Vascular function
dc.subject Ageing
dc.subject ACE-inhibition
dc.subject Bioactive peptides
dc.subject Ile-Pro-Pro
dc.subject VAL-PRO-PRO
dc.subject IN-VITRO
dc.subject B-2 RECEPTORS
dc.subject MILK
dc.subject 3111 Biomedicine
dc.subject 317 Pharmacy
dc.title Bradykinin -induced vasodilatation : Role of age, ACE1-inhibitory peptide, mas- and bradykinin receptors en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Department of Pharmacology
dc.contributor.organization Riitta Anneli Korpela / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.issn 0196-9781
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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