Internalization of a polysialic acid-binding Escherichia coli bacteriophage into eukaryotic neuroblastoma cells

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Lehti , T A , Pajunen , M I , Skog , M S & Finne , J 2017 , ' Internalization of a polysialic acid-binding Escherichia coli bacteriophage into eukaryotic neuroblastoma cells ' , Nature Communications , vol. 8 , 1915 . https://doi.org/10.1038/s41467-017-02057-3

Title: Internalization of a polysialic acid-binding Escherichia coli bacteriophage into eukaryotic neuroblastoma cells
Author: Lehti, Timo A.; Pajunen, Maria I.; Skog, Maria S.; Finne, Jukka
Contributor organization: Biosciences
Glycoscience Group
Research Programs Unit
Biochemistry and Biotechnology
Date: 2017-12-04
Language: eng
Number of pages: 12
Belongs to series: Nature Communications
ISSN: 2041-1723
DOI: https://doi.org/10.1038/s41467-017-02057-3
URI: http://hdl.handle.net/10138/229856
Abstract: Eukaryotic organisms are continuously exposed to bacteriophages, which are efficient gene transfer agents in bacteria. However, bacteriophages are considered not to pass the eukaryotic cell membrane and enter nonphagocytic cells. Here we report the binding and penetration of Escherichia coli PK1A2 bacteriophage into live eukaryotic neuroblastoma cells in vitro. The phage interacts with cell surface polysialic acid, which shares structural similarity with the bacterial phage receptor. Using fluorescence and electron microscopy, we show that phages are internalized via the endolysosomal route and persist inside the human cells up to one day without affecting cell viability. Phage capsid integrity is lost in lysosomes, and the phage DNA is eventually degraded. We did not detect the entry of phage DNA into the nucleus; however, we speculate that this might occur as a rare event, and propose that this potential mechanism could explain prokaryote-eukaryote gene flow.
Subject: HORIZONTAL GENE-TRANSFER
HUMAN GUT VIROME
ADHESION MOLECULE
PHAGE DISPLAY
TERMINAL PROTEINS
IN-VIVO
BACTERIAL
MIMICRY
BRAIN
ENDOSIALIDASE
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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