Heat Resistance Mediated by pLM58 Plasmid-Borne ClpL in Listeria monocytogenes

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dc.contributor.author Pöntinen, Anna
dc.contributor.author Aalto-Araneda, Mariella
dc.contributor.author Lindström, Miia
dc.contributor.author Korkeala, Hannu
dc.date.accessioned 2018-01-02T13:44:01Z
dc.date.available 2018-01-02T13:44:01Z
dc.date.issued 2017
dc.identifier.citation Pöntinen , A , Aalto-Araneda , M , Lindström , M & Korkeala , H 2017 , ' Heat Resistance Mediated by pLM58 Plasmid-Borne ClpL in Listeria monocytogenes ' , Msphere , vol. 2 , no. 6 , e00364-17 . https://doi.org/10.1128/mSphere.00364-17
dc.identifier.other PURE: 96166432
dc.identifier.other PURE UUID: b2eae1fd-4b10-4535-9c8e-a9a4f6744e25
dc.identifier.other WOS: 000417000900006
dc.identifier.other Scopus: 85041547809
dc.identifier.other ORCID: /0000-0002-7896-0680/work/40125219
dc.identifier.uri http://hdl.handle.net/10138/230108
dc.description.abstract Listeria monocytogenes is one of the most heat-resistant non-sporeforming food-borne pathogens and poses a notable risk to food safety, particularly when mild heat treatments are used in food processing and preparation. While general heat stress properties and response mechanisms of L. monocytogenes have been described, accessory mechanisms providing particular L. monocytogenes strains with the advantage of enhanced heat resistance are unknown. Here, we report plasmidmediated heat resistance of L. monocytogenes for the first time. This resistance is mediated by the ATP-dependent protease ClpL. We tested the survival of two wildtype L. monocytogenes strains-both of serotype 1/2c, sequence type ST9, and high sequence identity-at high temperatures and compared their genome composition in order to identify genetic mechanisms involved in their heat survival phenotype. L. monocytogenes AT3E was more heat resistant (0.0 CFU/ml log(10) reduction) than strain AL4E (1.4 CFU/ml log(10) reduction) after heating at 55 degrees C for 40 min. A prominent difference in the genome compositions of the two strains was a 58-kb plasmid (pLM58) harbored by the heat-resistant AT3E strain, suggesting plasmid-mediated heat resistance. Indeed, plasmid curing resulted in significantly decreased heat resistance (1.1 CFU/ml log(10) reduction) at 55 degrees C. pLM58 harbored a 2,115-bp open reading frame annotated as an ATP-dependent protease (ClpL)-encoding clpL gene. Introducing the clpL gene into a natively heat-sensitive L. monocytogenes strain (1.2 CFU/ml log(10) reduction) significantly increased the heat resistance of the recipient strain (0.4 CFU/ml log(10) reduction) at 55 degrees C. Plasmid-borne ClpL is thus a potential predictor of elevated heat resistance in L. monocytogenes. IMPORTANCE Listeria monocytogenes is a dangerous food pathogen causing the severe illness listeriosis that has a high mortality rate in immunocompromised individuals. Although destroyed by pasteurization, L. monocytogenes is among the most heat-resistant non-spore-forming bacteria. This poses a risk to food safety, as listeriosis is commonly associated with ready-to-eat foods that are consumed without thorough heating. However, L. monocytogenes strains differ in their ability to survive high temperatures, and comprehensive understanding of the genetic mechanisms underlying these differences is still limited. Whole-genome-sequence analysis and phenotypic characterization allowed us to identify a novel plasmid, designated pLM58, and a plasmid-borne ATP-dependent protease (ClpL), which mediated heat resistance in L. monocytogenes. As the first report on plasmid-mediated heat resistance in L. monocytogenes, our study sheds light on the accessory genetic mechanisms rendering certain L. monocytogenes strains particularly capable of surviving high temperatures-with plasmid-borne ClpL being a potential predictor of elevated heat resistance. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Msphere
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject ClpL
dc.subject Listeria
dc.subject heat stress
dc.subject heat tolerance
dc.subject plasmid-mediated resistance
dc.subject protease
dc.subject stress response
dc.subject BENZALKONIUM CHLORIDE RESISTANCE
dc.subject PHAGE SEARCH TOOL
dc.subject GENETIC-CHARACTERIZATION
dc.subject ANTIBIOTIC-RESISTANCE
dc.subject FOOD
dc.subject ACID
dc.subject TOLERANCE
dc.subject CADMIUM
dc.subject STRAINS
dc.subject STRESS
dc.subject 1183 Plant biology, microbiology, virology
dc.title Heat Resistance Mediated by pLM58 Plasmid-Borne ClpL in Listeria monocytogenes en
dc.type Article
dc.contributor.organization Departments of Faculty of Veterinary Medicine
dc.contributor.organization Food Hygiene and Environmental Health
dc.contributor.organization Miia Lindström / Principal Investigator
dc.contributor.organization Hannu Korkeala / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1128/mSphere.00364-17
dc.relation.issn 2379-5042
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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