Antileishmanial activity of extracts from Libidibia ferrea : development of in vitro and in vivo tests

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Comandolli-Wyrepkowski , C D , Jensen , B B , Grafova , I , dos Santos , P A , Comapa Barros , A M , Soares , F V , Marques Barcellos , J F , da Silva , A F , Grafov , A & Ramos Franco , A M 2017 , ' Antileishmanial activity of extracts from Libidibia ferrea : development of in vitro and in vivo tests ' , Acta Amazonica , vol. 47 , no. 4 , pp. 331-340 . https://doi.org/10.1590/1809-4392201700871

Title: Antileishmanial activity of extracts from Libidibia ferrea : development of in vitro and in vivo tests
Author: Comandolli-Wyrepkowski, Claudia Dantas; Jensen, Bruno Bezerra; Grafova, Iryna; dos Santos, Pierre Alexandre; Comapa Barros, Angela Maria; Soares, Fabiane Veloso; Marques Barcellos, Jose Fernando; da Silva, Aline Fagundes; Grafov, Andriy; Ramos Franco, Antonia Maria
Contributor organization: Department of Chemistry
Date: 2017
Language: eng
Number of pages: 10
Belongs to series: Acta Amazonica
ISSN: 0044-5967
DOI: https://doi.org/10.1590/1809-4392201700871
URI: http://hdl.handle.net/10138/231174
Abstract: Treatment of cutaneous leishmaniasis (CL) is difficult due to the scarce number of drugs able to eliminate completely the intracellular form of the parasite. In the present study, the aim was to evaluate: i) phytochemical properties of extracts from Libidibia ferrea; ii) antileishmanial activity of extracts from L. ferrea against promastigotes and amastigotes of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis; iii) the effects of topical treatment using hydrogel containing active extract of L. ferrea on golden hamsters infected with L. (L.) amazonensis. Extracts from leaves, branches and fruits of L. ferrea were obtained with hexane and methanol and were tested by in vitro assays in promastigotes and murine macrophages J774 experimentally infected with amastigotes of Leishmania. Groups of hamsters with CL received topical treatment with a formulation of extract (10%) hydrogels, 50 mg. day(-1) for 40 days. In vitro activity of FrMeOH (methanolic extract from fruits without seeds) resulted in significant reduction of viable promastigotes of L. (L.) amazonensis (IC50 of 15.4 mu g. mL(-1)) and demonstrated inhibition potential of amastigote forms of L. (L.) amazonensis and L. (V.) guyanensis and low cytotoxicity in macrophages. The overall data of topical treatment with extract hydrogels (GelFrMeOH) showed that lesion sizes were significantly reduced (42.78%), with low parasite burden by RT-qPCR and culture analysis by microscopy examination, and with histopathological findings such as lower inflammatory cell infiltration 40 days after treatment. Chemical analysis demonstrated FrMeOH contains high levels of phenolic compounds. The results indicate a possible alternative therapy for CL using phytotherapics. KEYWORDS: cutaneous leishmaniasis, natural products, topical formulations, experimental treatment
Subject: cutaneous leishmaniasis
natural products
topical formulations
experimental treatment
EXPERIMENTAL CUTANEOUS LEISHMANIASIS
CAESALPINIA-FERREA
TOPICAL TREATMENT
DRUG DEVELOPMENT
BALB/C MICE
ANTIOXIDANT
FRUITS
FORMULATION
TARGETS
MART
116 Chemical sciences
317 Pharmacy
3141 Health care science
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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