Chemical Profiling and Bioactivity of Body Wall Lipids from Strongylocentrotus droebachiensis

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http://hdl.handle.net/10138/231264

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Shikov , A N , Laakso , I , Pozharitskaya , O N , Seppänen-Laakso , T , Krishtopina , A S , Makarova , M N , Vuorela , H & Makarov , V 2017 , ' Chemical Profiling and Bioactivity of Body Wall Lipids from Strongylocentrotus droebachiensis ' , Marine Drugs , vol. 15 , no. 12 , 365 . https://doi.org/10.3390/md15120365

Title: Chemical Profiling and Bioactivity of Body Wall Lipids from Strongylocentrotus droebachiensis
Author: Shikov, Alexander N.; Laakso, Into; Pozharitskaya, Olga N.; Seppänen-Laakso, Tuulikki; Krishtopina, Anna S.; Makarova, Marina N.; Vuorela, Heikki; Makarov, Valery
Other contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Pharmaceutical Design and Discovery group



Date: 2017-12
Language: eng
Number of pages: 11
Belongs to series: Marine Drugs
ISSN: 1660-3397
DOI: https://doi.org/10.3390/md15120365
URI: http://hdl.handle.net/10138/231264
Abstract: The lipids from gonads and polyhydroxynaphthoquinone pigments from body walls of sea urchins are intensively studied. However, little is known about the body wall (BW) lipids. Ethanol extract (55 degrees C) contained about equal amounts of saturated (SaFA) and monounsaturated fatty acids (MUFA) representing 60% of total fatty acids, with myristic, palmitic and eicosenoic acids as major SaFAs and MUFAs, respectively. Non-methylene-interrupted dienes (13%) were composed of eicosadienoic and docosadienoic acids. Long-chain polyunsaturated fatty acids (LC-PUFA) included two main components, n6 arachidonic and n3 eicosapentaenoic acids, even with equal concentrations (15 mu g/mg) and a balanced n6/n3 PUFA ratio (0.86). The UPLC-ELSD analysis showed that a great majority of the lipids (80%) in the ethanolic extract were phosphatidylcholine (60 mu g/mg) and phosphatidylethanolamine (40 mu g/mg), while the proportion of neutral lipids remained lower than 20%. In addition, alkoxyglycerol derivativeschimyl, selachyl, and batyl alcoholswere quantified. We have assumed that the mechanism of action of body wall lipids in the present study is via the inhibition of MAPK p38, COX-1, and COX-2. Our findings open the prospective to utilize this lipid fraction as a source for the development of drugs with anti-inflammatory activity.
Subject: sea urchin
body wall lipids
inhibition of p38 MAPK
COX
GC-MS
UPLC-ELSD
POLYUNSATURATED FATTY-ACIDS
SEA-URCHIN
EICOSAPENTAENOIC ACID
GAS-CHROMATOGRAPHY
ENDOTHELIAL-CELLS
COX-2 EXPRESSION
DIETARY LIPIDS
BARENTS SEA
LIVER OIL
SHELLS
317 Pharmacy
116 Chemical sciences
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