Production of High Amounts of Hepatotoxin Nodularin and New Protease Inhibitors Pseudospumigins by the Brazilian Benthic Nostoc sp CENA543

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Jokela , J , Heinilä , L M P , Shishido , T K , Wahlsten , M , Fewer , D P , Fiore , M F , Wang , H , Haapaniemi , E , Permi , P & Sivonen , K 2017 , ' Production of High Amounts of Hepatotoxin Nodularin and New Protease Inhibitors Pseudospumigins by the Brazilian Benthic Nostoc sp CENA543 ' , Frontiers in Microbiology , vol. 8 , 1963 . https://doi.org/10.3389/fmicb.2017.01963

Title: Production of High Amounts of Hepatotoxin Nodularin and New Protease Inhibitors Pseudospumigins by the Brazilian Benthic Nostoc sp CENA543
Author: Jokela, Jouni; Heinilä, Lassi M. P.; Shishido, Tania K.; Wahlsten, Matti; Fewer, David P.; Fiore, Marli F.; Wang, Hao; Haapaniemi, Esa; Permi, Perttu; Sivonen, Kaarina
Contributor organization: University of Helsinki
Department of Food and Nutrition
Cyanobacteria research
Microbial Natural Products
Date: 2017-10-09
Language: eng
Number of pages: 14
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
DOI: https://doi.org/10.3389/fmicb.2017.01963
URI: http://hdl.handle.net/10138/231314
Abstract: Nostoc is a cyanobacterial genus, common in soils and a prolific producer of natural products. This research project aimed to explore and characterize Brazilian cyanobacteria for new bioactive compounds. Here we report the production of hepatotoxins and new protease inhibitors from benthic Nostoc sp. CENA543 isolated from a small, shallow, saline-alkaline lake in the Nhecolandia, Pantanal wetland area in Brazil. Nostoc sp. CENA543 produces exceptionally high amounts of nodularin-R. This is the first free-living Nostoc that produces nodularin at comparable levels as the toxic, bloom-forming, Nodularia spumigena. We also characterized pseudospumigins A-F, which are a novel family of linear tetrapeptides. Pseudospumigins are structurally related to linear tetrapeptide spumigins and aeruginosins both present in N. spumigena but differ in respect to their diagnostic amino acid, which is Ile/Leu/Val in pseudospumigins, Pro/mPro in spumigins, and Choi in aeruginosins. The pseudospumigin gene cluster is more similar to the spumigin biosynthetic gene cluster than the aeruginosin gene cluster. Pseudospumigin A inhibited trypsin (IC50 4.5 mu M after 1 h) in a similar manner as spumigin E from N. spumigena but was almost two orders of magnitude less potent. This study identifies another location and environment where the hepatotoxic nodularin has the potential to cause the death of eukaryotic organisms.
Subject: cyanobacteria
Nostoc
nodularin
spumigin
biosynthesis
SALINE-ALKALINE LAKES
CYANOBACTERIUM NODULARIA
BALTIC SEA
SPUMIGENA
PEPTIDES
DIVERSITY
TOXIN
SPECIFICITY
PREDICTION
EVOLUTION
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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