Microstructure analysis method for evaluating degenerated intervertebral disc tissue

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dc.contributor.author Friedmann, Andrea
dc.contributor.author Goehre , Felix
dc.contributor.author Ludtka, Christopher
dc.contributor.author Mendel, Thomas
dc.contributor.author Meisel, Hans-Joerg
dc.contributor.author Heilmann, Andreas
dc.contributor.author Schwan, Stefan
dc.date.accessioned 2018-01-21T22:08:28Z
dc.date.available 2021-12-17T18:48:28Z
dc.date.issued 2017-01
dc.identifier.citation Friedmann , A , Goehre , F , Ludtka , C , Mendel , T , Meisel , H-J , Heilmann , A & Schwan , S 2017 , ' Microstructure analysis method for evaluating degenerated intervertebral disc tissue ' , Micron , vol. 92 , pp. 51-62 . https://doi.org/10.1016/j.micron.2016.10.002
dc.identifier.other PURE: 78547262
dc.identifier.other PURE UUID: c3f1bdaf-adba-4d88-af63-89af1f9b83f1
dc.identifier.other WOS: 000390623600009
dc.identifier.other Scopus: 84996917468
dc.identifier.uri http://hdl.handle.net/10138/231344
dc.description.abstract Degeneration of intervertebral disc (IVD) tissue is characterized by several structural changes that result in variations in disc physiology and loss of biomechanical function. The complex process of degeneration exhibits highly intercorrelated biomechanical, biochemical, and cellular interactions. There is currently some understanding of the cellular changes in degenerated intervertebral disc tissue, but microstructural changes and deterioration of the tissue matrix has previously been rarely explored. In this work, sequestered IVD tissue was successfully characterized using histology, light microscopy, and scanning electron microscopy (SEM) to quantitatively evaluate parameters of interest for intervertebral disc degeneration (IDD) such as delamination of the collagenous matrix, cell density, cell size, and extra cellular matrix (ECM) thickness. Additional qualitative parameters investigated included matrix fibration and irregularity, neovascularization of the IVD, granular inclusions in the matrix, and cell cluster formation. The results of this study corroborated several previously published findings, including those positively correlating female gender and IVD cell density, age and cell size, and female gender and ECM thickness. Additionally, an array of quantitative and qualitative investigations of IVD degeneration could be successfully evaluated using the given methodology, resin-embedded SEM in particular. SEM is especially practical for studying micromorphological changes in tissue, as other microscopy methods can cause artificial tissue damage due to the preparation method. Investigation of the microstructural changes occurring in degenerated tissue provides a greater understanding of the complex process of disc degeneration as a whole. Developing a more complete picture of the degenerative changes taking place in the intervertebral disc is crucial for the advancement and application of regenerative therapies based on the pathology of intervertebral disc degeneration. (C) 2016 Elsevier Ltd. All rights reserved. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Micron
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Microstructure
dc.subject Disc tissue
dc.subject IDD
dc.subject Delamination
dc.subject Cell density
dc.subject Scanning electron microscopy
dc.subject SEM
dc.subject NUCLEUS PULPOSUS
dc.subject EXTRACELLULAR-MATRIX
dc.subject MECHANICAL FUNCTION
dc.subject SURGICAL SPECIMENS
dc.subject ANULUS FIBROSUS
dc.subject MOLECULAR-BASIS
dc.subject CROSS-LINKING
dc.subject AGE
dc.subject NEOVASCULARIZATION
dc.subject SENESCENCE
dc.subject 3126 Surgery, anesthesiology, intensive care, radiology
dc.title Microstructure analysis method for evaluating degenerated intervertebral disc tissue en
dc.type Article
dc.contributor.organization Neurokirurgian yksikkö
dc.contributor.organization Clinicum
dc.contributor.organization HUS Neurocenter
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.micron.2016.10.002
dc.relation.issn 0968-4328
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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