Assessing adverse effects of intra-articular botulinum toxin A in healthy Beagle dogs : A placebo-controlled, blinded, randomized trial

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Heikkilä , H M , Jokinen , T S , Syrjä , P , Junnila , J , Hielm-Björkman , A & Laitinen-Vapaavuori , O 2018 , ' Assessing adverse effects of intra-articular botulinum toxin A in healthy Beagle dogs : A placebo-controlled, blinded, randomized trial ' , PLoS One , vol. 13 , no. 1 , 0191043 . https://doi.org/10.1371/journal.pone.0191043

Title: Assessing adverse effects of intra-articular botulinum toxin A in healthy Beagle dogs : A placebo-controlled, blinded, randomized trial
Author: Heikkilä, Helka M.; Jokinen, Tarja S.; Syrjä, Pernilla; Junnila, Jouni; Hielm-Björkman, Anna; Laitinen-Vapaavuori, Outi
Contributor: University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Veterinary Pathology and Parasitology
University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
Date: 2018-01-10
Language: eng
Number of pages: 20
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/231560
Abstract: Objective To investigate the clinical, cytological, and histopathological adverse effects of intra-articularly injected botulinum toxin A in dogs and to study whether the toxin spreads from the joint after the injection. Methods A longitudinal, placebo-controlled, randomized clinical trial was conducted with six healthy laboratory Beagle dogs. Stifle joints were randomized to receive either 30 IU of onabotulinum toxin A or placebo in a 1: 1 ratio. Adverse effects and spread of the toxin were examined by evaluating dynamic and static weight-bearing of the injected limbs, by assessing painless range of motion and pain on palpation of joints, and by performing synovial fluid analysis, neurological examination, and electrophysiological recordings at different examination timepoints in a 12-week period after the injections. The dogs were then euthanized and autopsy and histopathological examination of joint structures and adjacent muscles and nerves were performed. Results Intra-articular botulinum toxin A did not cause local weakness or injection site pain. Instead, static weight-bearing and painless range of motion of stifle joints decreased in the placebo limbs. No clinically significant abnormalities associated with intra-articular botulinum toxin A were detected in the neurological examinations. Electrophysiological recordings showed low compound muscle action potentials in two dogs in the botulinum toxin A-injected limb. No significant changes were detected in the synovial fluid. Autopsy and histopathological examination of the joint and adjacent muscles and nerves did not reveal histopathological adverse effects of the toxin. Conclusion Intra-articular botulinum toxin A does not produce significant clinical, cytological, or histopathological adverse effects in healthy dogs. Based on the electrophysiological recordings, the toxin may spread from the joint, but its clinical impact seems to be low.
Subject: NERVE CONDUCTION-VELOCITY
UPPER-LIMB SPASTICITY
ARTHRITIS JOINT PAIN
PLATE GAIT ANALYSIS
SINGLE-FIBER EMG
KNEE OSTEOARTHRITIS
MUSCULAR WEAKNESS
REPEAT INJECTIONS
MUSCLE PARALYSIS
BRAIN-INJURY
413 Veterinary science
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