Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells

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Borger , J G , Morrison , V L , Filby , A , Garcia , C , Uotila , L M , Simbari , F , Fagerholm , S C & Zamoyska , R 2017 , ' Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells ' , Journal of Immunology , vol. 199 , no. 3 , pp. 874-884 . https://doi.org/10.4049/jimmunol.1700431

Title: Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells
Author: Borger, Jessica G.; Morrison, Vicky L.; Filby, Andrew; Garcia, Celine; Uotila, Liisa M.; Simbari, Fabio; Fagerholm, Susanna C.; Zamoyska, Rose
Contributor organization: Institute of Biotechnology
Integrins in immunity
Date: 2017-08-01
Language: eng
Number of pages: 11
Belongs to series: Journal of Immunology
ISSN: 0022-1767
DOI: https://doi.org/10.4049/jimmunol.1700431
URI: http://hdl.handle.net/10138/231799
Abstract: TCR stimulation by peptide-MHC complexes on APCs requires precise reorganization of molecules into the area of cellular contact to form an immunological synapse from where T cell signaling is initiated. Caveolin (Cav) 1, a widely expressed transmembrane protein, is involved in the regulation of membrane composition, cellular polarity and trafficking, and the organization of signal transduction pathways. The presence of Cav1 protein in T cells was identified only recently, and its function in this context is not well understood. We show that Cav1-knockout CD8 T cells have a reduction in membrane cholesterol and sphingomyelin, and upon TCR triggering they exhibit altered morphology and polarity, with reduced effector function compared with Cav1 wild-type CD8 T cells. In particular, redistribution of the b2 integrin LFA-1 to the immunological synapse is compromised in Cav1-knockout T cells, as is the ability of LFA-1 to form high-avidity interactions with ICAM-1. Our results identify a role for Cav1 in membrane organization and b2 integrin function in primary CD8 T cells.
Subject: FUNCTION-ASSOCIATED MOLECULE-1
LYMPHOCYTE FUNCTION
PLASMA-MEMBRANE
IN-VIVO
IMMUNOLOGICAL SYNAPSE
LFA-1-DEFICIENT MICE
ACTIN CYTOSKELETON
LEUKOCYTE ADHESION
INTEGRIN FUNCTION
PHORBOL ESTER
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Usage restriction: openAccess


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