Hokynar , K , Korhonen , S , Norja , P , Paavonen , J & Puolakkainen , M 2017 , ' Antibody to Chlamydia trachomatis proteins, TroA and HtrA, as a biomarker for Chlamydia trachomatis infection ' , European Journal of Clinical Microbiology & Infectious Diseases , vol. 36 , no. 1 , pp. 49-56 . https://doi.org/10.1007/s10096-016-2769-7
Title: | Antibody to Chlamydia trachomatis proteins, TroA and HtrA, as a biomarker for Chlamydia trachomatis infection |
Author: | Hokynar, K.; Korhonen, Suvi; Norja, P.; Paavonen, J.; Puolakkainen, M. |
Contributor organization: | Medicum Department of Virology Clinicum Department of Obstetrics and Gynecology Mirja Puolakkainen / Principal Investigator HUS Gynecology and Obstetrics HUSLAB |
Date: | 2017-01 |
Language: | eng |
Number of pages: | 8 |
Belongs to series: | European Journal of Clinical Microbiology & Infectious Diseases |
ISSN: | 0934-9723 |
DOI: | https://doi.org/10.1007/s10096-016-2769-7 |
URI: | http://hdl.handle.net/10138/231996 |
Abstract: | We studied whether antibody to two chlamydial proteins (TroA and HtrA) could be used as biomarkers of Chlamydia trachomatis infection. Methods: Recombinant proteins C. trachomatis TroA and HtrA were used as antigens in enzyme immunoassay (EIA). Both IgG and IgA antibody responses were studied. Results: IgG or IgA antibody to either protein was infrequently detected in sera from healthy blood donors or virgin girls. Patients attending the STI Clinic and patients with perihepatitis had often IgG antibody against TroA (25 and 50 % respectively) and HtrA (21 and 38 % respectively). Especially in sera from patients with chlamydial perihepatitis, the A(450nm) values with TroA were high (mean 1.591). A positive correlation between C. trachomatis MIF antibody and TroA (r = 0.7) as well as HtrA (r = 0.5) antibody was observed in sera from STI clinic patients and perihepatitis patients. Individuals with C. trachomatis infection and positive serology already when seeking medical attention had higher A(450nm) values for TroA (0.638) and HtrA (0.836) than patients with no marker of previous exposure or with no infection (0.208 and 0.234 respectively). Diagnosis of genital C. trachomatis infection is often NAAT-based, whereas serology has little value in testing for uncomplicated genital C. trachomatis infection. TroA and HtrA antibodies are potential biomarkers for evaluation of ascending and repeated C. trachomatis infection. |
Subject: |
TUBAL FACTOR INFERTILITY
IN-VITRO IRON RESTRICTION PERSISTENCE ANTIGENS PROTEASE IDENTIFICATION EXPRESSION PENICILLIN PATHOGEN 3121 General medicine, internal medicine and other clinical medicine 1183 Plant biology, microbiology, virology |
Peer reviewed: | Yes |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
Total number of downloads: Loading...
Files | Size | Format | View |
---|---|---|---|
Antibody_to_Chlamydia_trachomatis_proteins.pdf | 1.675Mb |
View/ |