The use of multiplex platforms for absolute and relative protein quantification of clinical material

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Christiansson , L , Mustjoki , S , Simonsson , B , Olsson-Strömberg , U , Loskog , A S I & Mangsbo , S M 2014 , ' The use of multiplex platforms for absolute and relative protein quantification of clinical material ' , EuPA Open Proteomics , vol. 3 , pp. 37 - 47 . https://doi.org/10.1016/j.euprot.2014.02.002

Title: The use of multiplex platforms for absolute and relative protein quantification of clinical material
Author: Christiansson, Lisa; Mustjoki, Satu; Simonsson, Bengt; Olsson-Strömberg, Ulla; Loskog, Angelica S.I.; Mangsbo, Sara M.
Contributor: University of Helsinki, Medicum
Date: 2014
Language: eng
Number of pages: 11
Belongs to series: EuPA Open Proteomics
ISSN: 2212-9685
URI: http://hdl.handle.net/10138/232493
Abstract: Abstract When introducing multiplex platforms to measure protein content in precious clinical material there is an increased risk of cross reactivity, loss of sensitivity as well as accuracy. In this paper, four multiplex platforms and one singleplex platform were compared by running pre- and post-treatment plasma samples from CML patients. We found a variation of absolute protein concentrations between platforms. For some of the analytes and platforms, relative differences between pre- and post-treatment samples correlated. We conclude that absolute concentrations measured by different platforms should be compared with caution and comparing relative differences could be more accurate.
Subject: Multiplex protein quantification, ELISA, Luminex, Somalogic, Meso Scale Discovery, Chronic myeloid leukemia
3111 Biomedicine
3121 General medicine, internal medicine and other clinical medicine
3122 Cancers
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