Chronic imaging through "transparent skull" in mice

Show full item record



Permalink

http://hdl.handle.net/10138/232825

Citation

Steinzeig , A , Molotkov , D & Castren , E 2017 , ' Chronic imaging through "transparent skull" in mice ' , PLoS One , vol. 12 , no. 8 , 0181788 . https://doi.org/10.1371/journal.pone.0181788

Title: Chronic imaging through "transparent skull" in mice
Author: Steinzeig, Anna; Molotkov, Dmitry; Castren, Eero
Contributor: University of Helsinki, Neuroscience Center
Date: 2017-08-16
Language: eng
Number of pages: 16
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/232825
Abstract: Growing interest in long-term visualization of cortical structure and function requires methods that allow observation of an intact cortex in longitudinal imaging studies. Here we describe a detailed protocol for the "transparent skull" (TS) preparation based on skull clearing with cyanoacrylate, which is applicable for long-term imaging through the intact skull in mice. We characterized the properties of the TS in imaging of intrinsic optical signals and compared them with the more conventional cranial window preparation. Our results show that TS is less invasive, maintains stabile transparency for at least two months, and compares favorably to data obtained from the conventional cranial window. We applied this method to experiments showing that a four-week treatment with the antidepressant fluoxetine combined with one week of monocular deprivation induced a shift in ocular dominance in the mouse visual cortex, confirming that fluoxetine treatment restores critical-period-like plasticity. Our results demonstrate that the TS preparation could become a useful method for long-term visualization of the living mouse brain.
Subject: CRANIAL WINDOW
INTRINSIC SIGNAL
CRITICAL PERIOD
THINNED-SKULL
LONG-TERM
PLASTICITY
CORTEX
MOUSE
VISION
EXPERIENCE
3112 Neurosciences
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
journal.pone.0181788.pdf 12.94Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record