Membrane-assisted viral DNA ejection

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Santos-Perez , I , Oksanen , H M , Bamford , D H , Goni , F M , Reguera , D & Abrescia , N G A 2017 , ' Membrane-assisted viral DNA ejection ' , Biochimica et Biophysica Acta. General Subjects , vol. 1861 , no. 3 , pp. 664-672 .

Title: Membrane-assisted viral DNA ejection
Author: Santos-Perez, Isaac; Oksanen, Hanna M.; Bamford, Dennis H.; Goni, Felix M.; Reguera, David; Abrescia, Nicola G. A.
Contributor organization: Biosciences
Institute of Biotechnology
Molecular Principles of Viruses
Structure of the Viral Universe
Date: 2017-03
Language: eng
Number of pages: 9
Belongs to series: Biochimica et Biophysica Acta. General Subjects
ISSN: 0304-4165
Abstract: Genome packaging and delivery are fundamental steps in the replication cycle of all viruses. Icosahedral viruses with linear double-stranded DNA (dsDNA) usually pacicage their genome into a preformed, rigid procapsid using the power generated by a virus-encoded packaging ATPase. The pressure and stored energy due to this confinement of DNA at a high density is assumed to drive the initial stages of genome ejection. Membrane-containing icosahedral viruses, such as bacteriophage PRD1, present an additional architectural complexity by enclosing their genome within an internal membrane vesicle. Upon adsorption to a host cell, the PRD1 membrane remodels into a proteo-lipidic tube that provides a conduit for passage of the ejected linear dsDNA through the cell envelope. Based on volume analyses of PRD1 membrane vesicles captured by cryo-electron tomography and modeling of the elastic properties of the vesicle, we propose that the internal membrane makes a crucial and active contribution during infection by maintaining the driving force for DNA ejection and countering the internal turgor pressure of the host These novel functions extend the role of the PRD1 viral membrane beyond tube formation or the mere physical confinement of the genome. The presence and assistance of an internal membrane might constitute a biological advantage that extends also to other viruses that package their linear dsDNA to high density within an internal vesicle. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Models of viral DNA packaging
Viral DNA delivery
Lipid-containing viruses
Membrane vesicle and properties
Biophysical modeling
Cryo-electron tomography
1183 Plant biology, microbiology, virology
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion

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